8RUF
Crystal structure of Rhizobium etli L-asparaginase ReAV D187A mutant
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | L-asparaginase II protein | polymer | 373 | 39510.9 | 4 | UniProt (Q2K0Z2) Pfam (PF06089) | Rhizobium etli | |
| 2 | E, GA, N, Y (A, D, B, C) | SULFATE ION | non-polymer | 96.1 | 4 | Chemie (SO4) | |||
| 3 | DA, EA, F, FA, G... (C, A, D, B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 21 | Chemie (EDO) | |||
| 4 | AA, H, NA, Q (C, A, D, B) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) | |||
| 5 | BA, I, R, TA (C, A, B, D) | CHLORIDE ION | non-polymer | 35.5 | 4 | Chemie (CL) | |||
| 6 | CA, M, PA, S, W (C, A, D, B) | GLYCEROL | non-polymer | 92.1 | 5 | Chemie (GOL) | |||
| 7 | OA, V (D, B) | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 2 | Chemie (TRS) | |||
| 8 | LA, QA (D) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 2 | Chemie (PEG) | |||
| 9 | UA, VA, WA, XA (A, B, C, D) | water | water | 18.0 | 1534 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 367 (UniProt: Q2K0Z2)
| PDB | External Database | Details |
|---|---|---|
| Gly -5 | - | expression tag |
| Ile -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Pro -2 | - | expression tag |
| Phe -1 | - | expression tag |
| Thr 0 | - | expression tag |
| Ala 187 | Asp 187 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 158043.4 | |
| Non-Polymers* | Number of molecules | 42 |
| Total formula weight | 3008.1 | |
| All* | Total formula weight | 161051.6 |
