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8RUA

Crystal structure of Rhizobium etli L-asparaginase ReAV C135A mutant

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A, B
(A, B)
L-asparaginase II proteinpolymer37339522.82UniProt (Q2K0Z2)
Pfam (PF06089)
Rhizobium etli
2C
(A)
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOLnon-polymer122.11Chemie (TRS)
3D, I, N
(A, B)
DI(HYDROXYETHYL)ETHERnon-polymer106.13Chemie (PEG)
4E, F, J, K, L...
(A, B)
GLYCEROLnon-polymer92.19Chemie (GOL)
5G, H, M, P, R...
(A, B)
1,2-ETHANEDIOLnon-polymer62.18Chemie (EDO)
6U
(B)
CHLORIDE IONnon-polymer35.51Chemie (CL)
7Y, Z
(A, B)
waterwater18.0440Chemie (HOH)
Sequence modifications
A, B: 1 - 367 (UniProt: Q2K0Z2)
PDBExternal DatabaseDetails
Gly -5-expression tag
Ile -4-expression tag
Asp -3-expression tag
Pro -2-expression tag
Phe -1-expression tag
Thr 0-expression tag
Ala 135Cys 135engineered mutation
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains2
Total formula weight79045.6
Non-Polymers*Number of molecules22
Total formula weight1801.3
All*Total formula weight80847.0
*Water molecules are not included.

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PDB entries from 2025-12-03

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