8RU6

Desulfovibrio desulfuricans [FeFe]-hydrogenase variant with both subunits linked by a 4 amino acid linker peptide derived from CpI of Clostridium pasteurianum

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Periplasmic [Fe] hydrogenase large subunit,CpI,Periplasmic [Fe] hydrogenase small subunit	polymer	491	54280.2	1	UniProt (P07598) UniProt (P29166) UniProt (P07603) Pfam (PF00037) Pfam (PF02906) Pfam (PF02256)	Desulfovibrio desulfuricans	Fe hydrogenlyase,CpI,Fe-only hydrogenase,[Fe] hydrogenase,Fe hydrogenlyase small chain
2	B, C, D, E (B)	TETRAETHYLENE GLYCOL	non-polymer		194.2	4	Chemie (PG4)
3	F, G, H (B)	IRON/SULFUR CLUSTER	non-polymer		351.6	3	Chemie (SF4)
4	I (B)	Binuclear [FeFe], di(thiomethyl)amine, carbon monoxide, cyanide cluster (-CO form)	non-polymer		383.0	1	Chemie (MHX)
5	J (B)	water	water		18.0	480	Chemie (HOH)

Sequence modifications

B: 1 - 392 (UniProt: P07598)
B: 393 - 396 (UniProt: P29166)
B: 397 - 481 (UniProt: P07603)

PDB	External Database	Details
Ser 482	-	expression tag
Ala 483	-	expression tag
Trp 484	-	expression tag
Ser 485	-	expression tag
His 486	-	expression tag
Pro 487	-	expression tag
Gln 488	-	expression tag
Phe 489	-	expression tag
Glu 490	-	expression tag
Lys 491	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	54280.2
Non-Polymers*	Number of molecules	8
	Total formula weight	2214.8
All*	Total formula weight	56495.0

*Water molecules are not included.