8RTB

Extended inner membrane complex (IMC) protomer structure (TrwM/VirB3-TrwK/VirB4-TrwI/VirB6-TrwG/VirB8-TrwE/VirB10) from the fully-assembled R388 type IV secretion system determined by cryo-EM.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	C	TrwM protein	polymer	104	12292.6	1	UniProt (O50329) Pfam (PF05101) In PDB	Escherichia coli
2	A, B	TrwK protein	polymer	823	93769.9	2	UniProt (O50330) Pfam (PF03135) Pfam (PF19044) In PDB	Escherichia coli
3	E, D, F, G	TrwG protein	polymer	231	25800.0	4	UniProt (O50335) Pfam (PF04335) In PDB	Escherichia coli
4	H	TrwE protein	polymer	395	42443.8	1	UniProt (O50337) Pfam (PF03743) In PDB	Escherichia coli
5	J	TrwI protein	polymer	342	35324.2	1	UniProt (O50333) Pfam (PF04610) In PDB	Escherichia coli

Sequence modifications

C: 1 - 104 (UniProt: O50329)

PDB	External Database	Details
Trp 24	Leu 24	conflict
Val 26	Glu 26	conflict

E, D, F, G: 1 - 231 (UniProt: O50335)

PDB	External Database	Details
Ala 188	Arg 188	conflict

H: 1 - 395 (UniProt: O50337)

PDB	External Database	Details
Asp 335	Asn 335	conflict

J: 1 - 342 (UniProt: O50333)

PDB	External Database	Details
Gln 108	Glu 108	conflict
Leu 152	Pro 152	conflict
Leu 153	Ala 153	conflict
Ala 154	Gly 154	conflict
Thr 155	Tyr 155	conflict
Leu 157	Pro 157	conflict
Leu 158	Ala 158	conflict
Ala 159	Gly 159	conflict

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	380800.4
All*	Total formula weight	380800.4

*Water molecules are not included.