8RSR

Crystal structure of marine actinobacteria clade rhodopsin (MAR) - human GTPase Arf1 (L8K,Q71L) chimera; N state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Microbial rhodopsin - GTPase chimera,ADP-ribosylation factor 1	polymer	408	45709.4	1	UniProt (P84077) Pfam (PF00025) UniProt (by SIFTS) (A0A7L2V2T6)	marine Actinobacteria clade
2	B (A)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP)
3	C (A)	EICOSANE	non-polymer		282.5	1	Chemie (LFA)
4	D (A)	OLEIC ACID	non-polymer		282.5	1	Chemie (OLA)
5	E (A)	RETINAL	non-polymer		284.4	1	Chemie (RET)	marine Actinobacteria clade
6	F (A)	water	water		18.0	107	Chemie (HOH)

Sequence modifications

A: 1 - 217 (PDB: 8RSR)
A: 218 - 397 (UniProt: P84077)

PDB	External Database	Details
Lys 224	Leu 8	engineered mutation
Leu 287	Gln 71	engineered mutation
Gly 398	-	expression tag
Ser 399	-	expression tag
Gly 400	-	expression tag
Gly 401	-	expression tag
Ser 402	-	expression tag
His 403	-	expression tag
His 404	-	expression tag
His 405	-	expression tag
His 406	-	expression tag
His 407	-	expression tag
His 408	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	45709.4
Non-Polymers*	Number of molecules	4
	Total formula weight	1292.6
All*	Total formula weight	47002.0

*Water molecules are not included.