8RRM
tripartite complex between 14-3-3 sigma, Fusicoccin-A, and a phosphopeptide optimized for a Fusicoccin-mediated stabilization of the complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | 14-3-3 protein sigma | polymer | 252 | 28137.5 | 2 | UniProt (P31947) Pfam (PF00244) | Homo sapiens (human) | Epithelial cell marker protein 1,Stratifin |
| 2 | C, D (C, D) | Phosphopeptide designed according to the sequence preferences favouring stabilisation by Fusicoccin-A | polymer | 11 | 1451.6 | 2 | synthetic construct | ||
| 3 | E, F (A, B) | FUSICOCCIN | non-polymer | 680.8 | 2 | Chemie (FSC) | |||
| 4 | G (B) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 5 | H (B) | BICARBONATE ION | non-polymer | 61.0 | 1 | Chemie (BCT) | |||
| 6 | I, J, K, L (A, B, C, D) | water | water | 18.0 | 206 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 248 (UniProt: P31947)
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Met -1 | - | expression tag |
| Ala 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 59178.1 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1458.1 | |
| All* | Total formula weight | 60636.2 |
