8RQK
Structure of the complete Vaccinia DNA-dependent RNA polymerase complex at 2.65A resolution
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA-directed RNA polymerase 147 kDa polypeptide | polymer | 1286 | 146995.7 | 1 | UniProt (P20504) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Vaccinia virus | |
| 2 | B (B) | DNA-directed RNA polymerase 133 kDa polypeptide | polymer | 1164 | 133526.9 | 1 | UniProt (P68694) Pfam (PF04560) Pfam (PF12415) Pfam (PF04565) Pfam (PF04567) Pfam (PF00562) | Vaccinia virus | |
| 3 | C (C) | DNA-directed RNA polymerase 35 kDa subunit | polymer | 305 | 35430.7 | 1 | UniProt (P21087) Pfam (PF03396) | Vaccinia virus | |
| 4 | D (E) | DNA-directed RNA polymerase 22 kDa subunit | polymer | 185 | 21365.7 | 1 | UniProt (P68608) Pfam (PF05273) | Vaccinia virus | |
| 5 | E (G) | DNA-directed RNA polymerase 18 kDa subunit | polymer | 161 | 17917.2 | 1 | UniProt (P21034) Pfam (PF03293) | Vaccinia virus | |
| 6 | F (I) | RNA polymerase-associated transcription-specificity factor RAP94 | polymer | 795 | 93667.6 | 1 | UniProt (P68439) Pfam (PF03294) | Vaccinia virus | Protein H4,RPO-associated protein of 94 kDa |
| 7 | G (J) | DNA-directed RNA polymerase 7 kDa subunit | polymer | 63 | 7299.7 | 1 | UniProt (P68315) | Vaccinia virus | |
| 8 | H (K) | Early transcription factor 82 kDa subunit | polymer | 710 | 82398.9 | 1 | UniProt (P20635) Pfam (PF04441) | Vaccinia virus | ETF large subunit,VETF A7 subunit,Vaccinia virus early transcription factor large subunit,VETF large subunit |
| 9 | I (L) | mRNA-capping enzyme regulatory subunit OPG124 | polymer | 287 | 33396.7 | 1 | UniProt (P20980) Pfam (PF03341) | Vaccinia virus | Virus termination factor small subunit,VTF small subunit,mRNA-capping enzyme 33 kDa subunit,mRNA-capping enzyme D12 subunit,mRNA-capping enzyme small subunit |
| 10 | J (O) | mRNA-capping enzyme catalytic subunit | polymer | 844 | 96888.8 | 1 | UniProt (P20979) Pfam (PF10640) Pfam (PF21004) Pfam (PF21005) Pfam (PF03291) | Vaccinia virus | Virus termination factor large subunit,VTF large subunit,mRNA-capping enzyme 97 kDa subunit,mRNA-capping enzyme D1 subunit,mRNA-capping enzyme large subunit |
| 11 | K, L (Q, R) | Core protein OPG073 | polymer | 129 | 14914.1 | 2 | UniProt (P68449) Pfam (PF06138) | Vaccinia virus | Protein E11 |
| 12 | M (S) | DNA-directed RNA polymerase 30 kDa polypeptide | polymer | 259 | 30074.3 | 1 | UniProt (P21082) Pfam (PF12410) Pfam (PF01096) | Vaccinia virus | |
| 13 | N (U) | URQ-UUG1-1 tRNA (72-MER) | polymer | 72 | 23136.7 | 1 | GenBank (2554165223) | Homo sapiens (human) | |
| 14 | O (Y) | Nucleoside triphosphatase I | polymer | 631 | 72465.3 | 1 | UniProt (P20637) Pfam (PF00176) Pfam (PF00271) Pfam (PF08469) | Vaccinia virus | Factor X,NPH-I,Nucleoside triphosphate phosphohydrolase I,NPH I |
| 15 | P (F) | DNA-directed RNA polymerase 19 kDa subunit | polymer | 164 | 19020.1 | 1 | UniProt (P68610) Pfam (PF05320) | Vaccinia virus | |
| 16 | Q, V, W, X (A, U) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
| 17 | R, S, T, U (A, B, I) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) | |||
| 18 | AA, BA, CA, DA, Y... (I, L, S, Y, A...) | water | water | 18.0 | 9 | Chemie (HOH) |
Sequence modifications
A: 1 - 1286 (UniProt: P20504)
B: 1 - 1164 (UniProt: P68694)
C: 1 - 305 (UniProt: P21087)
K: 1 - 710 (UniProt: P20635)
L: 1 - 287 (UniProt: P20980)
O: 1 - 844 (UniProt: P20979)
Y: 1 - 631 (UniProt: P20637)
| PDB | External Database | Details |
|---|---|---|
| Thr 258 | Ser 258 | variant |
| Glu 489 | Lys 489 | variant |
| Lys 1015 | Arg 1015 | variant |
| PDB | External Database | Details |
|---|---|---|
| Asn 6 | Asp 6 | variant |
| Phe 343 | Tyr 343 | variant |
| PDB | External Database | Details |
|---|---|---|
| Asn 236 | Asp 236 | variant |
| PDB | External Database | Details |
|---|---|---|
| Ala 133 | Val 133 | variant |
| PDB | External Database | Details |
|---|---|---|
| Asn 235 | Asp 235 | variant |
| PDB | External Database | Details |
|---|---|---|
| Phe 8 | Ser 8 | variant |
| Thr 202 | Lys 202 | variant |
| PDB | External Database | Details |
|---|---|---|
| Asp 329 | Asn 329 | variant |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 16 |
| Total formula weight | 843412.4 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 358.9 | |
| All* | Total formula weight | 843771.3 |
