8ROF

Human cohesin SMC1A-HD(shortCC-EQ)/RAD21-C complex - ADP-Mg-bound conformation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Structural maintenance of chromosomes protein 1A	polymer	366	40665.1	1	UniProt (Q14683) Pfam (PF02463)	Homo sapiens (human)	SMC protein 1A,SMC-1-alpha,SMC-1A,Sb1.8
2	B (B)	64-kDa C-terminal product	polymer	81	9203.6	1	UniProt (O60216) Pfam (PF04824)	Homo sapiens (human)	64-kDa carboxy-terminal product,65-kDa carboxy-terminal product
3	C (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
4	D (A)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
5	E, F (A, B)	water	water		18.0	302	Chemie (HOH)

Sequence modifications

A: 1 - 175 (UniProt: Q14683)
A: 1057 - 1233 (UniProt: Q14683)

PDB	External Database	Details
Gly 176	-	linker
Ser 177	-	linker
Gly 178	-	linker
Ser 179	-	linker
Leu 180	-	linker
Val 181	-	linker
Pro 182	-	linker
Arg 183	-	linker
Gly 1051	-	linker
Ser 1052	-	linker
Gly 1053	-	linker
Ser 1054	-	linker
Ala 1055	-	linker
Lys 1056	-	linker
Gln 1157	Glu 1157	engineered mutation

B: 558 - 629 (UniProt: O60216)

PDB	External Database	Details
Met 557	-	initiating methionine
Gly 630	-	expression tag
Ser 631	-	expression tag
Leu 632	-	expression tag
Glu 633	-	expression tag
Val 634	-	expression tag
Leu 635	-	expression tag
Phe 636	-	expression tag
Gln 637	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	49868.8
Non-Polymers*	Number of molecules	2
	Total formula weight	451.5
All*	Total formula weight	50320.3

*Water molecules are not included.