8ROB

Human cohesin SMC1A-HD(longCC-EQ)/RAD21-C complex - ATPgS-Mg-bound conformation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Structural maintenance of chromosomes protein 1A	polymer	456	51443.2	1	UniProt (Q14683) Pfam (PF02463)	Homo sapiens (human)	SMC protein 1A,SMC-1-alpha,SMC-1A,Sb1.8
2	B (B)	64-kDa C-terminal product	polymer	81	9203.6	1	UniProt (O60216) Pfam (PF04824)	Homo sapiens (human)	64-kDa carboxy-terminal product,65-kDa carboxy-terminal product
3	C (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
4	D (A)	PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER	non-polymer		523.2	1	Chemie (AGS)
5	E, F (A, B)	water	water		18.0	31	Chemie (HOH)

Sequence modifications

A: 1 - 977 (UniProt: Q14683)
A: 992 - 1233 (UniProt: Q14683)

PDB	External Database	Details
Glu 978	-	linker
Ser 979	-	linker
Ser 980	-	linker
Lys 981	-	linker
His 982	-	linker
Pro 983	-	linker
Thr 984	-	linker
Ser 985	-	linker
Leu 986	-	linker
Val 987	-	linker
Pro 988	-	linker
Arg 989	-	linker
Gly 990	-	linker
Ser 991	-	linker
Gln 1157	Glu 1157	engineered mutation

B: 558 - 629 (UniProt: O60216)

PDB	External Database	Details
Met 557	-	initiating methionine
Gly 630	-	expression tag
Ser 631	-	expression tag
Leu 632	-	expression tag
Glu 633	-	expression tag
Val 634	-	expression tag
Leu 635	-	expression tag
Phe 636	-	expression tag
Gln 637	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	60646.9
Non-Polymers*	Number of molecules	2
	Total formula weight	547.6
All*	Total formula weight	61194.4

*Water molecules are not included.