8RN0

Influenza polymerase A/H7N9-4M encapsidase plus 627(R) / human ANP32A (from "Influenza polymerase A/H7N9-4M replication complex" | Local refinement)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (C, F)	Polymerase basic protein 2	polymer	788	89037.6	2	UniProt (X5F427) Pfam (PF20947) Pfam (PF20948) Pfam (PF20949) Pfam (PF20950) Pfam (PF00604) Pfam (PF20951) Pfam (PF20952)	Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))
2	B (D)	Polymerase acidic protein	polymer	716	82829.6	1	UniProt (M9TI86) Pfam (PF00603)	Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))
3	C (E)	RNA-directed RNA polymerase catalytic subunit	polymer	757	86424.0	1	UniProt (S5ME50) Pfam (PF00602)	Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))
4	E (G)	Acidic leucine-rich nuclear phosphoprotein 32 family member A	polymer	275	31747.8	1	UniProt (P39687) Pfam (PF14580)	Homo sapiens (human)	Acidic nuclear phosphoprotein pp32,pp32,Leucine-rich acidic nuclear protein,LANP,Mapmodulin,Potent heat-stable protein phosphatase 2A inhibitor I1PP2A,Putative HLA-DR-associated protein I,PHAPI

Sequence modifications

C, F: 1 - 759 (UniProt: X5F427)

PDB	External Database	Details
Arg 74	Gly 74	engineered mutation
Gly 760	-	expression tag
Trp 761	-	expression tag
Ser 762	-	expression tag
His 763	-	expression tag
Pro 764	-	expression tag
Gln 765	-	expression tag
Phe 766	-	expression tag
Glu 767	-	expression tag
Lys 768	-	expression tag
Gly 769	-	expression tag
Gly 770	-	expression tag
Gly 771	-	expression tag
Ser 772	-	expression tag
Gly 773	-	expression tag
Gly 774	-	expression tag
Gly 775	-	expression tag
Ser 776	-	expression tag
Gly 777	-	expression tag
Gly 778	-	expression tag
Ser 779	-	expression tag
Ala 780	-	expression tag
Trp 781	-	expression tag
Ser 782	-	expression tag
His 783	-	expression tag
Pro 784	-	expression tag
Gln 785	-	expression tag
Phe 786	-	expression tag
Glu 787	-	expression tag
Lys 788	-	expression tag

D: 1 - 716 (UniProt: M9TI86)

PDB	External Database	Details
Lys 349	Glu 349	engineered mutation
Ile 490	Arg 490	engineered mutation

E: 1 - 757 (UniProt: S5ME50)

PDB	External Database	Details
Gly 577	Lys 577	engineered mutation

G: 1 - 249 (UniProt: P39687)

PDB	External Database	Details
Met -25	-	initiating methionine
Lys -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
Pro -17	-	expression tag
Met -16	-	expression tag
Ser -15	-	expression tag
Asp -14	-	expression tag
Tyr -13	-	expression tag
Asp -12	-	expression tag
Ile -11	-	expression tag
Pro -10	-	expression tag
Thr -9	-	expression tag
Thr -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	379076.5
All*	Total formula weight	379076.5

*Water molecules are not included.