8RMR

Influenza polymerase A/H7N9-4M replication complex, an asymmetric polymerase dimer bound to human ANP32A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	Polymerase acidic protein	polymer	716	82829.6	2	UniProt (M9TI86) Pfam (PF00603)	Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))
2	B, E (B, E)	RNA-directed RNA polymerase catalytic subunit	polymer	757	86424.0	2	UniProt (S5ME50) Pfam (PF00602)	Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))
3	C, F (C, F)	Polymerase basic protein 2	polymer	788	89037.6	2	UniProt (X5F427) Pfam (PF20947) Pfam (PF20948) Pfam (PF20949) Pfam (PF20950) Pfam (PF00604) Pfam (PF20951) Pfam (PF20952)	Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))
4	G (G)	Acidic leucine-rich nuclear phosphoprotein 32 family member A	polymer	275	31747.8	1	UniProt (P39687) Pfam (PF14580)	Homo sapiens (human)	Acidic nuclear phosphoprotein pp32,pp32,Leucine-rich acidic nuclear protein,LANP,Mapmodulin,Potent heat-stable protein phosphatase 2A inhibitor I1PP2A,Putative HLA-DR-associated protein I,PHAPI
5	H (B)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

A, D: 1 - 716 (UniProt: M9TI86)

PDB	External Database	Details
Lys 349	Glu 349	engineered mutation
Ile 490	Arg 490	engineered mutation

B, E: 1 - 757 (UniProt: S5ME50)

PDB	External Database	Details
Gly 577	Lys 577	engineered mutation

C, F: 1 - 759 (UniProt: X5F427)

PDB	External Database	Details
Arg 74	Gly 74	engineered mutation
Gly 760	-	expression tag
Trp 761	-	expression tag
Ser 762	-	expression tag
His 763	-	expression tag
Pro 764	-	expression tag
Gln 765	-	expression tag
Phe 766	-	expression tag
Glu 767	-	expression tag
Lys 768	-	expression tag
Gly 769	-	expression tag
Gly 770	-	expression tag
Gly 771	-	expression tag
Ser 772	-	expression tag
Gly 773	-	expression tag
Gly 774	-	expression tag
Gly 775	-	expression tag
Ser 776	-	expression tag
Gly 777	-	expression tag
Gly 778	-	expression tag
Ser 779	-	expression tag
Ala 780	-	expression tag
Trp 781	-	expression tag
Ser 782	-	expression tag
His 783	-	expression tag
Pro 784	-	expression tag
Gln 785	-	expression tag
Phe 786	-	expression tag
Glu 787	-	expression tag
Lys 788	-	expression tag

G: 1 - 249 (UniProt: P39687)

PDB	External Database	Details
Met -25	-	initiating methionine
Lys -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
Pro -17	-	expression tag
Met -16	-	expression tag
Ser -15	-	expression tag
Asp -14	-	expression tag
Tyr -13	-	expression tag
Asp -12	-	expression tag
Ile -11	-	expression tag
Pro -10	-	expression tag
Thr -9	-	expression tag
Thr -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	548330.2
Non-Polymers*	Number of molecules	1
	Total formula weight	24.3
All*	Total formula weight	548354.5

*Water molecules are not included.