8RMF

Structure of the core ISC complex under turnover conditions (FDX2-bound in proximal conformation)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, F (A, E)	Isoform Mitochondrial of Cysteine desulfurase	polymer	404	45165.6	2	UniProt (Q9Y697) Pfam (PF00266)	Homo sapiens (human)
2	B, G (B, F)	LYR motif-containing protein 4	polymer	115	13502.4	2	UniProt (Q9HD34) Pfam (PF05347)	Homo sapiens (human)
3	C, I (C, G)	Acyl carrier protein	polymer	78	8645.5	2	UniProt (P0A6A8) Pfam (PF00550)	Escherichia coli BL21(DE3)	ACP,Cytosolic-activating factor,CAF,Fatty acid synthase acyl carrier protein
4	D, H (D, H)	Isoform 1 of Iron-sulfur cluster assembly enzyme ISCU	polymer	143	15684.1	2	UniProt (Q9H1K1) Pfam (PF01592)	Homo sapiens (human)	NifU-like N-terminal domain-containing protein,NifU-like protein
5	E (I)	Ferredoxin-2, mitochondrial	polymer	121	13344.0	1	UniProt (Q6P4F2) Pfam (PF00111)	Homo sapiens (human)	Adrenodoxin-like protein,Ferredoxin-1-like protein
6	J, N (C, G)	S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate	non-polymer		540.7	2	Chemie (8Q1)
7	K, M (D, H)	FE (II) ION	non-polymer		55.8	2	Chemie (FE2)
8	L (I)	FE2/S2 (INORGANIC) CLUSTER	non-polymer		175.8	1	Chemie (FES)
9	O, P, Q, R, S... (A, B, D, I, E...)	water	water		18.0	253	Chemie (HOH)

Sequence modifications

A, E: 56 - 457 (UniProt: Q9Y697)

PDB	External Database	Details
Met 54	-	initiating methionine
Ser 55	-	expression tag

B, F: 1 - 91 (UniProt: Q9HD34)

PDB	External Database	Details
Met -23	-	initiating methionine
Gly -22	-	expression tag
Ser -21	-	expression tag
Ser -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
Gly -13	-	expression tag
Ser -12	-	expression tag
Pro -11	-	expression tag
Thr -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
His -1	-	expression tag
Asn 0	-	expression tag
Ala 11	Ser 11	conflict

D, H: 35 - 167 (UniProt: Q9H1K1)

PDB	External Database	Details
Met 33	-	initiating methionine
Ala 34	-	expression tag
Leu 168	-	expression tag
Glu 169	-	expression tag
His 170	-	expression tag
His 171	-	expression tag
His 172	-	expression tag
His 173	-	expression tag
His 174	-	expression tag
His 175	-	expression tag

I: 68 - 186 (UniProt: Q6P4F2)

PDB	External Database	Details
Met 66	-	initiating methionine
Ala 67	-	expression tag
Ser 68	Gly 68	conflict

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	179339.1
Non-Polymers*	Number of molecules	5
	Total formula weight	1368.8
All*	Total formula weight	180707.9

*Water molecules are not included.