8RJN
Serial femtosecond X-ray structure of a fluorescence optimized bathy phytochrome PAiRFP2 derived from wild-type Agp2 in its Pfr state (I0b).
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | histidine kinase | polymer | 509 | 56678.6 | 2 | UniProt (A9CI81) Pfam (PF08446) Pfam (PF01590) Pfam (PF00360) | Agrobacterium fabrum str. C58 | |
| 2 | C, E (A, B) | 3-[(2Z)-2-({3-(2-carboxyethyl)-5-[(E)-(4-ethenyl-3-methyl-5-oxo-1,5-dihydro-2H-pyrrol-2-ylidene)methyl]-4-methyl-1H-pyrrol-2-yl}methylidene)-5-{(Z)-[(3E,4S)-3-ethylidene-4-methyl-5-oxopyrrolidin-2-ylidene]methyl}-4-methyl-2H-pyrrol-3-yl]propanoic acid | non-polymer | 584.7 | 2 | Chemie (EL5) | |||
| 3 | D, F, G (A, B) | SULFATE ION | non-polymer | 96.1 | 3 | Chemie (SO4) | |||
| 4 | H, I (A, B) | water | water | 18.0 | 216 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 505 (UniProt: A9CI81)
| PDB | External Database | Details |
|---|---|---|
| Arg 69 | Lys 69 | engineered mutation |
| Lys 83 | Arg 83 | engineered mutation |
| Asp 120 | Gly 120 | engineered mutation |
| Thr 123 | Ala 123 | engineered mutation |
| Leu 163 | Met 163 | engineered mutation |
| Glu 168 | Gln 168 | engineered mutation |
| Pro 220 | Arg 220 | engineered mutation |
| Asn 243 | Ser 243 | engineered mutation |
| Phe 244 | Val 244 | engineered mutation |
| Asp 269 | Gly 269 | engineered mutation |
| Val 276 | Ala 276 | engineered mutation |
| Cys 280 | Tyr 280 | engineered mutation |
| Ala 294 | Glu 294 | engineered mutation |
| Phe 303 | His 303 | engineered mutation |
| Arg 333 | His 333 | engineered mutation |
| Leu 336 | Ile 336 | engineered mutation |
| Arg 349 | Asp 349 | engineered mutation |
| Ile 351 | Met 351 | engineered mutation |
| Val 386 | Ala 386 | engineered mutation |
| Asp 409 | Gly 409 | engineered mutation |
| Ile 419 | Leu 419 | engineered mutation |
| Ser 469 | Thr 469 | engineered mutation |
| Thr 487 | Ala 487 | engineered mutation |
| Gly 494 | Glu 494 | engineered mutation |
| Leu 506 | - | expression tag |
| Glu 507 | - | expression tag |
| Tyr 508 | - | expression tag |
| Lys 509 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 113357.2 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 1457.5 | |
| All* | Total formula weight | 114814.7 |
