8RIS
Computationally redesigend variant of Pyrrolysyl-tRNA Synthetase (Y306A)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Pyrrolysine--tRNA ligase | polymer | 270 | 31074.6 | 1 | UniProt (Q8PWY1) Pfam (PF01409) | Methanosarcina mazei | Pyrrolysine--tRNA(Pyl) ligase,Pyrrolysyl-tRNA synthetase,PylRS |
| 2 | B (A) | PENTAETHYLENE GLYCOL | non-polymer | 238.3 | 1 | Chemie (1PE) | |||
| 3 | C (A) | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 4 | D, E (A) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 5 | F, G, H (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 3 | Chemie (EDO) | |||
| 6 | I (A) | water | water | 18.0 | 65 | Chemie (HOH) |
Sequence modifications
A: 188 - 454 (UniProt: Q8PWY1)
| PDB | External Database | Details |
|---|---|---|
| Gly 185 | - | expression tag |
| Ser 186 | - | expression tag |
| His 187 | - | expression tag |
| Ala 306 | Tyr 306 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 31074.6 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 631.7 | |
| All* | Total formula weight | 31706.3 |
