8RIF
Cryo-EM structure of the MCM double hexamer loaded onto dsDNA.
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, I (2, A) | DNA replication licensing factor MCM2 | polymer | 868 | 98911.5 | 2 | UniProt (P29469) Pfam (PF12619) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) | Saccharomyces cerevisiae (brewer's yeast) | Minichromosome maintenance protein 2 |
| 2 | B, J (3, B) | DNA replication licensing factor MCM3 | polymer | 1006 | 111720.2 | 2 | UniProt (P24279) Pfam (PF22207) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) | Saccharomyces cerevisiae (brewer's yeast) | Minichromosome maintenance protein 3 |
| 3 | C, K (4, C) | DNA replication licensing factor MCM4 | polymer | 933 | 105138.4 | 2 | UniProt (P30665) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF21128) | Saccharomyces cerevisiae (brewer's yeast) | Cell division control protein 54 |
| 4 | D, L (5, D) | Minichromosome maintenance protein 5 | polymer | 775 | 86505.7 | 2 | UniProt (P29496) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF21933) | Saccharomyces cerevisiae (brewer's yeast) | Cell division control protein 46 |
| 5 | E, M (6, E) | DNA replication licensing factor MCM6 | polymer | 1017 | 113110.2 | 2 | UniProt (P53091) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF18263) | Saccharomyces cerevisiae (brewer's yeast) | Minichromosome maintenance protein 6 |
| 6 | F, N (7, F) | DNA replication licensing factor MCM7 | polymer | 845 | 95049.9 | 2 | UniProt (P38132) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) | Saccharomyces cerevisiae (brewer's yeast) | Cell division control protein 47,Minichromosome maintenance protein 7 |
| 7 | G (X) | DNA (53-MER) | polymer | 53 | 16326.4 | 1 | Saccharomyces cerevisiae | ||
| 8 | H (Y) | DNA (53-MER) | polymer | 53 | 16335.5 | 1 | Saccharomyces cerevisiae | ||
| 9 | BA, O (A, 2) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 2 | Chemie (ATP) | |||
| 10 | CA, FA, IA, MA, P... (A, B, D, F, 2...) | MAGNESIUM ION | non-polymer | 24.3 | 8 | Chemie (MG) | |||
| 11 | AA, DA, GA, JA, KA... (7, A, C, D, E...) | ZINC ION | non-polymer | 65.4 | 10 | Chemie (ZN) | |||
| 12 | EA, HA, LA, R, U... (B, D, F, 3, 5...) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 6 | Chemie (ADP) |
Sequence modifications
3, B: 1 - 971 (UniProt: P24279)
| PDB | External Database | Details |
|---|---|---|
| Met -34 | - | initiating methionine |
| Lys -33 | - | expression tag |
| Arg -32 | - | expression tag |
| Arg -31 | - | expression tag |
| Trp -30 | - | expression tag |
| Lys -29 | - | expression tag |
| Lys -28 | - | expression tag |
| Asn -27 | - | expression tag |
| Phe -26 | - | expression tag |
| Ile -25 | - | expression tag |
| Ala -24 | - | expression tag |
| Val -23 | - | expression tag |
| Ser -22 | - | expression tag |
| Ala -21 | - | expression tag |
| Ala -20 | - | expression tag |
| Asn -19 | - | expression tag |
| Arg -18 | - | expression tag |
| Phe -17 | - | expression tag |
| Lys -16 | - | expression tag |
| Lys -15 | - | expression tag |
| Ile -14 | - | expression tag |
| Ser -13 | - | expression tag |
| Ser -12 | - | expression tag |
| Ser -11 | - | expression tag |
| Gly -10 | - | expression tag |
| Ala -9 | - | expression tag |
| Leu -8 | - | expression tag |
| Glu -7 | - | expression tag |
| Asn -6 | - | expression tag |
| Leu -5 | - | expression tag |
| Tyr -4 | - | expression tag |
| Phe -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Glu 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 14 |
| Total formula weight | 1253533.9 | |
| Non-Polymers* | Number of molecules | 26 |
| Total formula weight | 4426.1 | |
| All* | Total formula weight | 1257959.9 |
