8RHI

Lytic Transglycosylase MltD of Pseudomonas aeruginosa in a ternary complex bound to Bulgecin A and chito-tetraose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	LysM peptidoglycan-binding domain-containing protein	polymer	338	38579.4	2	UniProt (A0A0C7CWY9) Pfam (PF01464) Pfam (PF01476) In PDB	Pseudomonas aeruginosa	Membrane-bound lytic murein transglycosylase D,Murein transglycosylase
2	D	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
3	E	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		830.8	1	In PDB GlyTouCan (G01361SX)
4	A, B	4-O-(4-O-SULFONYL-N-ACETYLGLUCOSAMININYL)-5-METHYLHYDROXY-L-PROLINE-TAURINE	non-polymer		552.6	2	Chemie (BLG)
5	A, B	ZINC ION	non-polymer		65.4	9	Chemie (ZN)
6		water	water		18.0	186	Chemie (HOH)

Sequence modifications

A, B: 76 - 393 (UniProt: A0A0C7CWY9)

PDB	External Database	Details
Met 56	-	initiating methionine
Gly 57	-	expression tag
Ser 58	-	expression tag
Ser 59	-	expression tag
His 60	-	expression tag
His 61	-	expression tag
His 62	-	expression tag
His 63	-	expression tag
His 64	-	expression tag
His 65	-	expression tag
Ser 66	-	expression tag
Ser 67	-	expression tag
Gly 68	-	expression tag
Glu 69	-	expression tag
Asn 70	-	expression tag
Leu 71	-	expression tag
Tyr 72	-	expression tag
Phe 73	-	expression tag
Gln 74	-	expression tag
Gly 75	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	77158.8
Branched	Number of molecules	2
	Total formula weight	1255.2
Non-Polymers*	Number of molecules	11
	Total formula weight	1693.8
All*	Total formula weight	80107.8

*Water molecules are not included.