8REW
CryoEM structure of human GARP-lTGFbeta1 in complex with a Fab fragment derived from an activating antibody.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Transforming growth factor beta-1 | polymer | 390 | 44383.1 | 4 | UniProt (P01137) Pfam (PF00688) Pfam (PF00019) | Homo sapiens (human) | TGF-beta-1 |
| 2 | E (E) | Transforming growth factor beta activator LRRC32 | polymer | 674 | 73261.5 | 1 | UniProt (Q14392) Pfam (PF01462) Pfam (PF13855) | Homo sapiens (human) | Garpin,Glycoprotein A repetitions predominant,GARP,Leucine-rich repeat-containing protein 32 |
| 3 | F, I (F, I) | hFab LHT-22, Light Chain | polymer | 238 | 25104.0 | 2 | Lama glama | ||
| 4 | G, H (G, H) | hFab LHT-22, Heavy Chain | polymer | 247 | 26268.6 | 2 | Lama glama | ||
| 5 | J, K (J, K) | alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 748.7 | 2 | In PDB GlyTouCan (G22573RC) | |||
| 6 | L (L) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G42666HT) | |||
| 7 | M, N, O, P, Q (A, C, E) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 5 | Chemie (NAG) |
Sequence modifications
E: 1 - 628 (UniProt: Q14392)
| PDB | External Database | Details |
|---|---|---|
| Glu 629 | - | expression tag |
| Ala 630 | - | expression tag |
| Ala 631 | - | expression tag |
| Ala 632 | - | expression tag |
| Glu 633 | - | expression tag |
| Asn 634 | - | expression tag |
| Leu 635 | - | expression tag |
| Tyr 636 | - | expression tag |
| Phe 637 | - | expression tag |
| Gln 638 | - | expression tag |
| Gly 639 | - | expression tag |
| Ala 640 | - | expression tag |
| Ala 641 | - | expression tag |
| Trp 642 | - | expression tag |
| Ser 643 | - | expression tag |
| His 644 | - | expression tag |
| Pro 645 | - | expression tag |
| Gln 646 | - | expression tag |
| Phe 647 | - | expression tag |
| Glu 648 | - | expression tag |
| Lys 649 | - | expression tag |
| Gly 650 | - | expression tag |
| Ala 651 | - | expression tag |
| Ala 652 | - | expression tag |
| Trp 653 | - | expression tag |
| Ser 654 | - | expression tag |
| His 655 | - | expression tag |
| Pro 656 | - | expression tag |
| Gln 657 | - | expression tag |
| Phe 658 | - | expression tag |
| Glu 659 | - | expression tag |
| Lys 660 | - | expression tag |
| Gly 661 | - | expression tag |
| Ala 662 | - | expression tag |
| Ala 663 | - | expression tag |
| Trp 664 | - | expression tag |
| Ser 665 | - | expression tag |
| His 666 | - | expression tag |
| Pro 667 | - | expression tag |
| Gln 668 | - | expression tag |
| Phe 669 | - | expression tag |
| Glu 670 | - | expression tag |
| Lys 671 | - | expression tag |
| Gly 672 | - | expression tag |
| Ala 673 | - | expression tag |
| Ala 674 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 353539.0 | |
| Branched | Number of molecules | 3 |
| Total formula weight | 1921.8 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 1106.0 | |
| All* | Total formula weight | 356566.8 |
