8REE
Cryo-EM structure of bacterial RNA polymerase-sigma54 initial transcribing complex - 9nt complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | DNA-directed RNA polymerase subunit alpha | polymer | 321 | 35726.8 | 2 | UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Escherichia coli K-12 | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C (C) | DNA-directed RNA polymerase subunit beta | polymer | 1341 | 150691.8 | 1 | UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) | Escherichia coli K-12 | |
| 3 | D (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1373 | 152040.3 | 1 | UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Escherichia coli K-12 | |
| 4 | E (E) | DNA-directed RNA polymerase subunit omega | polymer | 74 | 8449.5 | 1 | UniProt (P0A800) Pfam (PF01192) | Escherichia coli K-12 | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | F (M) | RNA polymerase sigma-54 factor | polymer | 329 | 36725.5 | 1 | Pfam (PF04963) Pfam (PF04552) UniProt (by SIFTS) (P06223) | Klebsiella oxytoca | |
| 6 | G (N) | DNA (45-MER) | polymer | 45 | 13794.8 | 1 | Klebsiella oxytoca | ||
| 7 | H (R) | RNA (5'-R(P*GP*CP*CP*GP*CP*GP*AP*UP*C)-3') | polymer | 9 | 2846.8 | 1 | Klebsiella oxytoca | ||
| 8 | I (T) | DNA (49-MER) | polymer | 49 | 15165.7 | 1 | Klebsiella oxytoca | ||
| 9 | J (D) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 10 | K, L (D) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 451167.9 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 155.1 | |
| All* | Total formula weight | 451323.0 |
