8REC
Cryo-EM structure of bacterial RNA polymerase-sigma54 initial transcribing complex - 7nt complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | N | DNA (46-MER) | polymer | 46 | 14059.0 | 1 | Klebsiella oxytoca | ||
| 2 | R | RNA (5'-R(P*GP*CP*CP*GP*CP*GP*A)-3') | polymer | 7 | 2235.4 | 1 | Klebsiella oxytoca | ||
| 3 | T | DNA (51-MER) | polymer | 51 | 15800.1 | 1 | Klebsiella oxytoca | ||
| 4 | A, B | DNA-directed RNA polymerase subunit alpha | polymer | 321 | 35726.8 | 2 | UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB | Escherichia coli K-12 | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 5 | C | DNA-directed RNA polymerase subunit beta | polymer | 1341 | 150691.8 | 1 | UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) In PDB | Escherichia coli K-12 | |
| 6 | D | DNA-directed RNA polymerase subunit beta' | polymer | 1373 | 152040.3 | 1 | UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB | Escherichia coli K-12 | |
| 7 | E | DNA-directed RNA polymerase subunit omega | polymer | 74 | 8449.5 | 1 | UniProt (P0A800) Pfam (PF01192) In PDB | Escherichia coli K-12 | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 8 | M | RNA polymerase sigma-54 factor | polymer | 347 | 39010.9 | 1 | Pfam (PF04963) Pfam (PF04552) UniProt (by SIFTS) (P06223) In PDB | Klebsiella oxytoca | |
| 9 | D | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 10 | D | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 453740.6 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 155.1 | |
| All* | Total formula weight | 453895.7 |
