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8REA

Cryo-EM structure of bacterial RNA polymerase-sigma54 initial transcribing complex - 5nt post-translocated complex

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A, B
(A, B)
DNA-directed RNA polymerase subunit alphapolymer32135726.82UniProt (P0A7Z4)Escherichia coli K-12RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2C
(C)
DNA-directed RNA polymerase subunit betapolymer1341150691.81UniProt (P0A8V2)Escherichia coli K-12
3D
(D)
DNA-directed RNA polymerase subunit beta'polymer1373152040.31UniProt (P0A8T7)Escherichia coli K-12
4E
(E)
DNA-directed RNA polymerase subunit omegapolymer748449.51UniProt (P0A800)Escherichia coli K-12RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5F
(M)
RNA polymerase sigma-54 factorpolymer38042688.01Klebsiella oxytoca
6G
(N)
DNA (44-MER)polymer4513817.91Klebsiella oxytoca
7H
(R)
RNA (5'-R(P*GP*CP*CP*GP*C)-3')polymer51561.01Klebsiella oxytoca
8I
(T)
DNA (51-MER)polymer5115775.11Klebsiella oxytoca
9J
(D)
MAGNESIUM IONnon-polymer24.31Chemie (MG)
10K, L
(D)
ZINC IONnon-polymer65.42Chemie (ZN)
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains9
Total formula weight456477.1
Non-Polymers*Number of molecules3
Total formula weight155.1
All*Total formula weight456632.2
*Water molecules are not included.

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PDB entries from 2025-07-09

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