8RE5

Aspartyl/Asparaginyl beta-hydroxylase (AspH) R735Q variant in complex with Mn, 2-oxosuberate and a Factor X derived peptide fragment

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Aspartyl/asparaginyl beta-hydroxylase	polymer	444	51164.4	1	UniProt (Q12797) Pfam (PF13432) Pfam (PF05118)	Homo sapiens (human)	Aspartate beta-hydroxylase,ASP beta-hydroxylase,Peptide-aspartate beta-dioxygenase
2	B (B)	Coagulation factor X	polymer	39	4190.4	1	UniProt (P00742) Pfam (PF00008)	synthetic construct
3	C, D (A)	2-oxidanylideneoctanedioic acid	non-polymer		188.2	2	Chemie (YQX)
4	E (A)	MANGANESE (II) ION	non-polymer		54.9	1	Chemie (MN)
5	F (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
6	G, H (A, B)	water	water		18.0	448	Chemie (HOH)

Sequence modifications

A: 315 - 758 (UniProt: Q12797)

PDB	External Database	Details
Gln 735	Arg 735	engineered mutation

B: 86 - 124 (UniProt: P00742)

PDB	External Database	Details
Ser 90	Cys 90	engineered mutation
Ser 95	Cys 95	engineered mutation
Ser 112	Cys 112	engineered mutation
Ser 121	Cys 121	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	55354.8
Non-Polymers*	Number of molecules	4
	Total formula weight	466.7
All*	Total formula weight	55821.5

*Water molecules are not included.