8RD3

Crystal structure of Saccharomyces cerevisiae Nmd4 protein bound to Upf1 helicase domain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	ATP-dependent helicase NAM7	polymer	640	72303.0	1	UniProt (P30771) Pfam (PF18141) Pfam (PF13086) Pfam (PF13087) In PDB	Saccharomyces cerevisiae S288C
2	B	Nonsense-mediated decay protein 4	polymer	221	25560.1	1	UniProt (Q12129) In PDB	Saccharomyces cerevisiae S288C
3	A, B	1,2-ETHANEDIOL	non-polymer		62.1	9	Chemie (EDO)
4	A	SUCCINIC ACID	non-polymer		118.1	1	Chemie (SIN)
5	A	FORMIC ACID	non-polymer		46.0	1	Chemie (FMT)
6	A	MALONIC ACID	non-polymer		104.1	1	Chemie (MLA)
7		water	water		18.0	80	Chemie (HOH)

Sequence modifications

A: 221 - 851 (UniProt: P30771)

PDB	External Database	Details
Met 218	-	initiating methionine
Ala 219	-	expression tag
Ser 220	-	expression tag
His 852	-	expression tag
His 853	-	expression tag
His 854	-	expression tag
His 855	-	expression tag
His 856	-	expression tag
His 857	-	expression tag

B: 2 - 218 (UniProt: Q12129)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	97863.1
Non-Polymers*	Number of molecules	12
	Total formula weight	826.8
All*	Total formula weight	98689.9

*Water molecules are not included.