8RB8
Cryo-EM structure of the NADH:ferredoxin oxidoreductase RNF from Azotobacter vinelandii, purified with 2-ME/TCEP, NADH added
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Ion-translocating oxidoreductase complex subunit A | polymer | 190 | 19942.1 | 1 | UniProt (C1DMA8) Pfam (PF02508) | Azotobacter vinelandii DJ | Rnf electron transport complex subunit A |
| 2 | B (C) | Ion-translocating oxidoreductase complex subunit C | polymer | 496 | 52234.2 | 1 | UniProt (C1DMA6) Pfam (PF13375) Pfam (PF01512) Pfam (PF10531) Pfam (PF13237) | Azotobacter vinelandii DJ | Rnf electron transport complex subunit C |
| 3 | C (D) | Ion-translocating oxidoreductase complex subunit D | polymer | 366 | 39189.0 | 1 | UniProt (C1DMA5) Pfam (PF03116) | Azotobacter vinelandii DJ | Rnf electron transport complex subunit D |
| 4 | D (E) | Ion-translocating oxidoreductase complex subunit E | polymer | 238 | 25576.3 | 1 | UniProt (Q9F5Y1) Pfam (PF02508) | Azotobacter vinelandii DJ | Rnf electron transport complex subunit E |
| 5 | E (G) | Ion-translocating oxidoreductase complex subunit G | polymer | 237 | 25932.7 | 1 | UniProt (C1DMA4) Pfam (PF04205) | Azotobacter vinelandii DJ | Rnf electron transport complex subunit G |
| 6 | F (H) | Protein RnfH | polymer | 86 | 9644.9 | 1 | UniProt (Q9F5Y0) Pfam (PF03658) | Azotobacter vinelandii DJ | |
| 7 | G (B) | Ion-translocating oxidoreductase complex subunit B | polymer | 174 | 17704.6 | 1 | UniProt (C1DMA7) Pfam (PF04060) Pfam (PF14697) | Azotobacter vinelandii DJ | Rnf electron transport complex subunit B |
| 8 | H (A) | FE2/S2 (INORGANIC) CLUSTER | non-polymer | 175.8 | 1 | Chemie (FES) | |||
| 9 | I, M, N, P, Q... (A, D, E) | DODECYL-BETA-D-MALTOSIDE | non-polymer | 510.6 | 13 | Chemie (LMT) | |||
| 10 | AA, J, V (G, C, D) | FLAVIN MONONUCLEOTIDE | non-polymer | 456.3 | 3 | Chemie (FMN) | Azotobacter vinelandii DJ | ||
| 11 | BA, CA, K, L (B, C) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 4 | Chemie (SF4) | |||
| 12 | O (D) | RIBOFLAVIN | non-polymer | 376.4 | 1 | Chemie (RBF) |
Sequence modifications
G: 1 - 229 (UniProt: C1DMA4)
| PDB | External Database | Details |
|---|---|---|
| Trp 230 | - | expression tag |
| Ser 231 | - | expression tag |
| His 232 | - | expression tag |
| Pro 233 | - | expression tag |
| Gln 234 | - | expression tag |
| Phe 235 | - | expression tag |
| Glu 236 | - | expression tag |
| Lys 237 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 7 |
| Total formula weight | 190223.8 | |
| Non-Polymers* | Number of molecules | 22 |
| Total formula weight | 9965.8 | |
| All* | Total formula weight | 200189.6 |
