8RAZ
The crystal structure of DNA-bound human MutSbeta (MSH2_E749A/MSH3_E976A) in the canonical mismatch bound conformation with ADP bound in MSH2 and MSH3
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA mismatch repair protein Msh2 | polymer | 934 | 104803.8 | 1 | UniProt (P43246) Pfam (PF01624) Pfam (PF05188) Pfam (PF05192) Pfam (PF05190) Pfam (PF00488) | Homo sapiens (human) | |
| 2 | B (B) | DNA mismatch repair protein Msh3 | polymer | 1137 | 127576.7 | 1 | UniProt (P20585) Pfam (PF01624) Pfam (PF05188) Pfam (PF05192) Pfam (PF00488) | Homo sapiens (human) | |
| 3 | C (C) | DNA (5'-D(P*AP*TP*CP*TP*GP*AP*AP*GP*CP*CP*GP*AP*TP*CP*GP*AP*TP*GP*G)-3') | polymer | 24 | 7345.7 | 1 | synthetic construct | ||
| 4 | D (D) | DNA (5'-D(P*CP*AP*TP*CP*GP*AP*TP*CP*GP*CP*AP*GP*CP*TP*TP*CP*AP*GP*A)-3') | polymer | 24 | 7369.8 | 1 | synthetic construct | ||
| 5 | E, H (A, B) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 2 | Chemie (ADP) | |||
| 6 | F (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 7 | G, K (A, B) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 8 | I, J (B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 2 | Chemie (EDO) | |||
| 9 | L, M, N (A, B, D) | water | water | 18.0 | 61 | Chemie (HOH) |
Sequence modifications
A: 1 - 934 (UniProt: P43246)
B: 1 - 1137 (UniProt: P20585)
| PDB | External Database | Details |
|---|---|---|
| Ala 749 | Glu 749 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Val 79 | Ile 79 | conflict |
| Arg 949 | Gln 949 | conflict |
| Ala 976 | Glu 976 | engineered mutation |
| Thr 1045 | Ala 1045 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 247096.0 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 1141.5 | |
| All* | Total formula weight | 248237.6 |
