8R9V

CryoEM structure of the primed actomyosin-5a complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Unconventional myosin-Va	polymer	801	92247.9	1	UniProt (Q99104) Pfam (PF00063) Pfam (PF00612)	Mus musculus (house mouse)	Dilute myosin heavy chain,non-muscle
2	B, C, D (B, C, D)	Actin, alpha skeletal muscle	polymer	376	41888.7	3	UniProt (P68135) Pfam (PF00022)	Oryctolagus cuniculus (rabbit)	Alpha-actin-1
3	E (A)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
4	F, I, K, M (A, B, C, D)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
5	G, H, J, L (A, B, C, D)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	4	Chemie (ADP)

Sequence modifications

A: 1 - 797 (UniProt: Q99104)

PDB	External Database	Details
Ala 217	Ser 217	engineered mutation
-	Asp 594	deletion
-	Asp 595	deletion
-	Glu 596	deletion
-	Lys 597	deletion
Asp 798	-	expression tag
Tyr 799	-	expression tag
Lys 800	-	expression tag
Asp 801	-	expression tag
Asp 802	-	expression tag
Asp 803	-	expression tag
Asp 804	-	expression tag
Lys 805	-	expression tag

B, C, D: 1 - 376 (UniProt: P68135)

PDB	External Database	Details
Ace 1	-	acetylation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	217913.8
Non-Polymers*	Number of molecules	9
	Total formula weight	1901.0
All*	Total formula weight	219814.8

*Water molecules are not included.