8R87

Cryo-EM structure of the Sars-Cov2 S trimer without RBDs

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Spike glycoprotein,Fibritin	polymer	1059	116805.1	3	UniProt (P0DTC2) UniProt (P10104) Pfam (PF16451) Pfam (PF19209) Pfam (PF01601)	Severe acute respiratory syndrome coronavirus	S glycoprotein,E2,Peplomer protein,Collar protein,Whisker antigen control protein

Sequence modifications

A, B, C: 1 - 331 (UniProt: P0DTC2)
A, B, C: 530 - 1208 (UniProt: P0DTC2)

PDB	External Database	Details
Gly 332	-	linker
Ser 333	-	linker
Gly 334	-	linker
Ser 335	-	linker
Gly 336	-	linker
Ser 337	-	linker
Gly 682	Arg 682	engineered mutation
Ser 683	Arg 683	engineered mutation
Ser 685	Arg 685	engineered mutation
Pro 817	Phe 817	engineered mutation
Pro 892	Ala 892	engineered mutation
Pro 899	Ala 899	engineered mutation
Pro 942	Ala 942	engineered mutation
Pro 986	Lys 986	engineered mutation
Pro 987	Val 987	engineered mutation
Gly 1209	-	linker
Ser 1210	-	linker

A, B, C: 1211 - 1237 (UniProt: P10104)

PDB	External Database	Details
Leu 1232	Phe 479	engineered mutation
Gly 1238	-	expression tag
Arg 1239	-	expression tag
Ser 1240	-	expression tag
Gly 1241	-	expression tag
Ser 1242	-	expression tag
Gly 1243	-	expression tag
His 1244	-	expression tag
His 1245	-	expression tag
His 1246	-	expression tag
His 1247	-	expression tag
His 1248	-	expression tag
His 1249	-	expression tag
His 1250	-	expression tag
His 1251	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	350415.3
All*	Total formula weight	350415.3

*Water molecules are not included.