8R2P

YZwIdeal x16 a scaffold for cryo-EM of small proteins of interest crystallizing in space group 19 (P 21 21 21)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Putrescine aminotransferase,Immunoglobulin G-binding protein A	polymer	523	57054.1	4	UniProt (P42588) UniProt (P38507) Pfam (PF00202) Pfam (PF02216) UniProt (by SIFTS) (P02976)	Escherichia coli	PAT,PATase,Cadaverine transaminase,Diamine transaminase,Putrescine transaminase,Putrescine--2-oxoglutaric acid transaminase,Putrescine:2-OG aminotransferase,IgG-binding protein A,Staphylococcal protein A,SpA
2	E, F, G, H (A, B, C, D)	PYRIDOXAL-5'-PHOSPHATE	non-polymer		247.1	4	Chemie (PLP)
3	I, J, K, L (A, B, C, D)	water	water		18.0	1507	Chemie (HOH)

Sequence modifications

A, B, C, D: 7 - 457 (UniProt: P42588)

PDB	External Database	Details
Gly -6	-	expression tag
Ser -5	-	expression tag
Ser -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
His 2	-	expression tag
Tyr 3	-	expression tag
Tyr 4	-	expression tag
Leu 5	-	expression tag
Glu 6	-	expression tag
Glu 458	-	linker
Ala 459	-	linker
Leu 460	-	linker
Glu 461	-	linker
Val 462	-	linker
Ser 463	-	linker
Ala 464	-	linker

A, B, C, D: 465 - 516 (UniProt: P38507)

PDB	External Database	Details
Ala 487	Gly 240	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	228216.5
Non-Polymers*	Number of molecules	4
	Total formula weight	988.6
All*	Total formula weight	229205.1

*Water molecules are not included.