8R2M
Mycobacterium smegnatis RNA polymerase transcription initiation complex with SigmaA, RbpA, HelD N-terminal domain and an upstream-fork promoter fragment; State III conformation
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | DNA-directed RNA polymerase subunit alpha | polymer | 350 | 37959.4 | 2 | UniProt (A0QSL8) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Mycolicibacterium smegmatis MC2 155 | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C (C) | DNA-directed RNA polymerase subunit beta | polymer | 1169 | 128680.1 | 1 | UniProt (P60281) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) | Mycolicibacterium smegmatis MC2 155 | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | D (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1317 | 146712.9 | 1 | UniProt (A0QS66) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Mycolicibacterium smegmatis MC2 155 | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | E (F) | RNA polymerase sigma factor SigA | polymer | 466 | 51573.6 | 1 | UniProt (A0QW02) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) | Mycolicibacterium smegmatis MC2 155 | |
| 5 | F (H) | Helicase | polymer | 736 | 81298.1 | 1 | UniProt (I7G5V9) Pfam (PF00580) UniProt (by SIFTS) (A0QUE0) | Mycolicibacterium smegmatis MC2 155 | |
| 6 | G (J) | RNA polymerase-binding protein RbpA | polymer | 114 | 13078.7 | 1 | UniProt (A0QZ11) Pfam (PF13397) | Mycolicibacterium smegmatis MC2 155 | |
| 7 | H (O) | DNA 31-MER | polymer | 31 | 9565.2 | 1 | Mycolicibacterium smegmatis MC2 155 | ||
| 8 | I (P) | DNA 22-MER | polymer | 22 | 6685.4 | 1 | Mycolicibacterium smegmatis MC2 155 | ||
| 9 | J (E) | DNA-directed RNA polymerase subunit omega | polymer | 107 | 11544.8 | 1 | UniProt (A0QWT1) Pfam (PF01192) | Mycolicibacterium smegmatis MC2 155 | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 10 | K, L (D) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 11 | M (D) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 525057.6 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 155.1 | |
| All* | Total formula weight | 525212.7 |
