8R17

Crystal structure of Neurospora crassa NADase with modified C-terminus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Conidial surface nicotinamide adenine dinucleotide glycohydrolase,Conidial surface nicotinamide adenine dinucleotide glycohydrolase nadA	polymer	259	28183.3	1	UniProt (Q7S936) UniProt (Q4WL81) Pfam (PF14021)	Neurospora crassa	NAD(P)ase,NADase,Diphosphopyridine nucleotidase,DPNase,NAD(+) hydrolase,NADP(+) hydrolase,NADase,NAD(+) hydrolase nadA,NADP(+) hydrolase nadA
2	B (B)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
3	C, D (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
4	E, F (A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5	G (A)	water	water		18.0	23	Chemie (HOH)

Sequence modifications

A: 1 - 232 (UniProt: Q7S936)
A: 233 - 254 (UniProt: Q4WL81)

PDB	External Database	Details
Asp 255	-	expression tag
Val 256	-	expression tag
Leu 257	-	expression tag
Phe 258	-	expression tag
Gln 259	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	28183.3
Branched	Number of molecules	1
	Total formula weight	424.4
Non-Polymers*	Number of molecules	4
	Total formula weight	626.6
All*	Total formula weight	29234.3

*Water molecules are not included.