8QZE
Heme-domain BM3 variant 21B3_F87V-A328F
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Bifunctional cytochrome P450/NADPH--P450 reductase | polymer | 466 | 53274.7 | 2 | UniProt (P14779) Pfam (PF00067) In PDB | Priestia megaterium | |
2 | A, B | PROTOPORPHYRIN IX CONTAINING FE | non-polymer | 616.5 | 2 | Chemie (HEM) | |||
3 | A, B | IMIDAZOLE | non-polymer | 69.1 | 2 | Chemie (IMD) | |||
4 | A | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
5 | water | water | 18.0 | 270 | Chemie (HOH) |
Sequence modifications
A, B: 0 - 463 (UniProt: P14779)
PDB | External Database | Details |
---|---|---|
Val 58 | Ile 59 | engineered mutation |
Val 87 | Phe 88 | engineered mutation |
Arg 100 | His 101 | engineered mutation |
Leu 107 | Phe 108 | engineered mutation |
Ser 135 | Ala 136 | engineered mutation |
Val 145 | Met 146 | engineered mutation |
His 239 | Asn 240 | engineered mutation |
Thr 274 | Ser 275 | engineered mutation |
Phe 328 | Ala 329 | engineered mutation |
Glu 434 | Lys 435 | engineered mutation |
Ile 446 | Val 447 | engineered mutation |
Leu 464 | - | expression tag |
Glu 465 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 106549.4 | |
Non-Polymers* | Number of molecules | 5 |
Total formula weight | 1463.2 | |
All* | Total formula weight | 108012.6 |