8QZE

Heme-domain BM3 variant 21B3_F87V-A328F

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Bifunctional cytochrome P450/NADPH--P450 reductase	polymer	466	53274.7	2	UniProt (P14779) Pfam (PF00067)	Priestia megaterium
2	C, F (A, B)	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	2	Chemie (HEM)
3	D, G (A, B)	IMIDAZOLE	non-polymer		69.1	2	Chemie (IMD)
4	E (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
5	H, I (A, B)	water	water		18.0	270	Chemie (HOH)

Sequence modifications

A, B: 0 - 463 (UniProt: P14779)

PDB	External Database	Details
Val 58	Ile 59	engineered mutation
Val 87	Phe 88	engineered mutation
Arg 100	His 101	engineered mutation
Leu 107	Phe 108	engineered mutation
Ser 135	Ala 136	engineered mutation
Val 145	Met 146	engineered mutation
His 239	Asn 240	engineered mutation
Thr 274	Ser 275	engineered mutation
Phe 328	Ala 329	engineered mutation
Glu 434	Lys 435	engineered mutation
Ile 446	Val 447	engineered mutation
Leu 464	-	expression tag
Glu 465	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	106549.4
Non-Polymers*	Number of molecules	5
	Total formula weight	1463.2
All*	Total formula weight	108012.6

*Water molecules are not included.