8QYN

Human 20S proteasome assembly intermediate structure 5

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Proteasome subunit alpha type-2	polymer	234	25927.5	1	UniProt (P25787) Pfam (PF10584) Pfam (PF00227)	Homo sapiens (human)	Macropain subunit C3,Multicatalytic endopeptidase complex subunit C3,Proteasome component C3
2	B (B)	Proteasome subunit alpha type-4	polymer	261	29525.8	1	UniProt (P25789) Pfam (PF10584) Pfam (PF00227)	Homo sapiens (human)	Macropain subunit C9,Multicatalytic endopeptidase complex subunit C9,Proteasome component C9,Proteasome subunit L
3	C (C)	Proteasome subunit alpha type-7	polymer	248	27929.9	1	UniProt (O14818) Pfam (PF10584) Pfam (PF00227)	Homo sapiens (human)	Proteasome subunit RC6-1,Proteasome subunit XAPC7
4	D (D)	Proteasome subunit alpha type-5	polymer	242	26462.0	1	UniProt (P28066) Pfam (PF10584) Pfam (PF00227)	Homo sapiens (human)	Macropain zeta chain,Multicatalytic endopeptidase complex zeta chain,Proteasome zeta chain
5	E (E)	Proteasome subunit alpha type-1	polymer	264	29621.7	1	UniProt (P25786) Pfam (PF00227) Pfam (PF10584)	Homo sapiens (human)
6	F (F)	Proteasome subunit alpha type-3	polymer	255	28469.3	1	UniProt (P25788) Pfam (PF10584) Pfam (PF00227)	Homo sapiens (human)	Macropain subunit C8,Multicatalytic endopeptidase complex subunit C8,Proteasome component C8
7	G (G)	Proteasome subunit alpha type-6	polymer	246	27432.5	1	UniProt (P60900) Pfam (PF10584) Pfam (PF00227)	Homo sapiens (human)	27 kDa prosomal protein,p27K,Macropain iota chain,Multicatalytic endopeptidase complex iota chain,Proteasome iota chain
8	H (H)	Proteasome maturation protein	polymer	142	15831.0	1	UniProt (Q9Y244) Pfam (PF05348)	Homo sapiens (human)
9	I (I)	Proteasome assembly chaperone 1	polymer	288	32891.9	1	UniProt (O95456) Pfam (PF16094)	Homo sapiens (human)	PAC-1,Chromosome 21 leucine-rich protein,C21-LRP,Down syndrome critical region protein 2
10	J (J)	Proteasome assembly chaperone 2	polymer	265	29449.1	1	UniProt (Q969U7) Pfam (PF09754)	Homo sapiens (human)
11	K (K)	Proteasome subunit beta type-7	polymer	332	35490.4	1	UniProt (Q99436) Pfam (PF00227) Pfam (PF12465)	Homo sapiens (human)
12	L (L)	Proteasome subunit beta type-3	polymer	205	22972.9	1	UniProt (P49720) Pfam (PF00227)	Homo sapiens (human)	Proteasome chain 13,Proteasome component C10-II,Proteasome theta chain
13	M (M)	Proteasome subunit beta type-2	polymer	202	22890.3	1	UniProt (P49721) Pfam (PF00227)	Homo sapiens (human)
14	N (N)	Proteasome subunit beta type-5	polymer	264	28536.3	1	UniProt (P28074) Pfam (PF00227)	Homo sapiens (human)
15	O (O)	Proteasome subunit beta type-1	polymer	241	26522.4	1	UniProt (P20618) Pfam (PF00227)	Homo sapiens (human)	Macropain subunit C5,Multicatalytic endopeptidase complex subunit C5,Proteasome component C5,Proteasome gamma chain
16	P (P)	Proteasome subunit beta type-4	polymer	264	29219.1	1	UniProt (P28070) Pfam (PF00227)	Homo sapiens (human)	26 kDa prosomal protein,HsBPROS26,PROS-26,Macropain beta chain,Multicatalytic endopeptidase complex beta chain,Proteasome beta chain,Proteasome chain 3,HsN3
17	Q (Q)	Proteasome subunit beta type-6	polymer	239	25335.6	1	UniProt (P28072) Pfam (PF00227)	Homo sapiens (human)

Sequence modifications

D: 1 - 241 (UniProt: P28066)

PDB	External Database	Details
Ace 0	-	acetylation

E: 1 - 263 (UniProt: P25786)

PDB	External Database	Details
Ace 0	-	acetylation

H: 1 - 141 (UniProt: Q9Y244)

PDB	External Database	Details
Ace 0	-	acetylation

J: 1 - 264 (UniProt: Q969U7)

PDB	External Database	Details
Ace 0	-	acetylation

K: 1 - 238 (UniProt: Q99436)

PDB	External Database	Details
Glu 238	-	expression tag
Asp 238	-	expression tag
Leu 238	-	expression tag
Tyr 238	-	expression tag
Phe 238	-	expression tag
Gln 238	-	expression tag
Ser 238	-	expression tag
Val 238	-	expression tag
Asp 238	-	expression tag
Ser 238	-	expression tag
Ala 238	-	expression tag
Trp 238	-	expression tag
Ser 239	-	expression tag
His 239	-	expression tag
Pro 239	-	expression tag
Gln 239	-	expression tag
Phe 239	-	expression tag
Glu 239	-	expression tag
Lys 239	-	expression tag
Gly 239	-	expression tag
Gly 239	-	expression tag
Gly 239	-	expression tag
Ser 239	-	expression tag
Gly 239	-	expression tag
Gly 239	-	expression tag
Gly 239	-	expression tag
Ser 239	-	expression tag
Gly 239	-	expression tag
Gly 239	-	expression tag
Ser 239	-	expression tag
Ala 239	-	expression tag
Trp 239	-	expression tag
Ser 239	-	expression tag
His 239	-	expression tag
Pro 239	-	expression tag
Gln 239	-	expression tag
Phe 239	-	expression tag
Glu 239	-	expression tag
Lys 240	-	expression tag
Unk 247	-	expression tag
Unk 248	-	expression tag
Unk 249	-	expression tag
Unk 250	-	expression tag
Unk 251	-	expression tag
Unk 252	-	expression tag
Unk 253	-	expression tag
Unk 254	-	expression tag
Unk 255	-	expression tag
Unk 256	-	expression tag
Unk 257	-	expression tag
Unk 258	-	expression tag
Unk 259	-	expression tag
Unk 260	-	expression tag
Unk 261	-	expression tag
Unk 262	-	expression tag

M: 1 - 201 (UniProt: P49721)

PDB	External Database	Details
Ace 0	-	acetylation

N: 1 - 263 (UniProt: P28074)

PDB	External Database	Details
Ace 0	-	acetylation

P: 1 - 264 (UniProt: P28070)

PDB	External Database	Details
Thr 234	Ile 234	variant

Q: 1 - 239 (UniProt: P28072)

PDB	External Database	Details
Gly 145	Val 145	variant

Contents of the asymmetric unit

Polymers	Number of chains	17
	Total formula weight	464507.6
All*	Total formula weight	464507.6

*Water molecules are not included.