8QUE

Structure of the Bacteriophage PhiKZ non-virion RNA Polymerase bound to DNA and RNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	PHIKZ055	polymer	508	57976.6	1	UniProt (Q8SDA7) In PDB	Pseudomonas phage phiKZ
2	C	DNA-directed RNA polymerase	polymer	700	78780.5	1		Pseudomonas phage phiKZ
3	D	PHIKZ074	polymer	677	77513.5	1	UniProt (Q8SD88) In PDB	Pseudomonas phage phiKZ
4	E	PHIKZ123	polymer	543	62959.1	1	UniProt (Q8SD39) In PDB	Pseudomonas phage phiKZ
5	F	DNA	polymer	75	23098.9	1		Pseudomonas phage phiKZ
6	G	DNA	polymer	75	23058.9	1	GenBank (29134936)	Pseudomonas phage phiKZ
7	H	RNA	polymer	8	2589.6	1		Pseudomonas phage phiKZ
8	B	PHIKZ068	polymer	521	59419.8	1	UniProt (Q8SD94) In PDB	Pseudomonas phage phiKZ
9	I, J	DNA	polymer	17	3016.7	2		Pseudomonas phage phiKZ
10	A, D	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A: 1 - 415 (UniProt: Q8SDA7)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

A: 416 - 488 (PDB: 8QUE)
E: 1 - 543 (UniProt: Q8SD39)

PDB	External Database	Details
Gly 197	Asp 197	variant

B: 1 - 521 (UniProt: Q8SD94)

PDB	External Database	Details
Glu 2	Gln 2	variant

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	391430.1
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	391560.9

*Water molecules are not included.