8QP5

Release Complex: BAM bound EspP (SurA released)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Outer membrane protein assembly factor BamA	polymer	790	88530.8	1	UniProt (P0A940) Pfam (PF07244) Pfam (PF01103)	Escherichia coli	Omp85
2	B (B)	Outer membrane protein assembly factor BamB	polymer	373	39882.4	1	UniProt (P77774) Pfam (PF13360)	Escherichia coli
3	C (C)	Outer membrane protein assembly factor BamC	polymer	320	34401.3	1	UniProt (P0A903) Pfam (PF06804)	Escherichia coli
4	D (D)	Outer membrane protein assembly factor BamD	polymer	226	25816.8	1	UniProt (P0AC02) Pfam (PF13525)	Escherichia coli
5	E (E)	Outer membrane protein assembly factor BamE	polymer	104	11610.8	1	UniProt (P0A937) Pfam (PF04355)	Escherichia coli
6	F (P)	Chaperone SurA,Serine protease EspP	polymer	814	89181.3	1	UniProt (P0ABZ6) UniProt (Q7BSW5) Pfam (PF09312) Pfam (PF00639) Pfam (PF03797)	Escherichia coli	Peptidyl-prolyl cis-trans isomerase SurA,PPIase SurA,Rotamase SurA,Survival protein A

Sequence modifications

A: 21 - 810 (UniProt: P0A940)

PDB	External Database	Details
Cys 425	Ser 425	engineered mutation

E: 20 - 113 (UniProt: P0A937)

PDB	External Database	Details
Gly 114	-	expression tag
Gly 115	-	expression tag
His 116	-	expression tag
His 117	-	expression tag
His 118	-	expression tag
His 119	-	expression tag
His 120	-	expression tag
His 121	-	expression tag
His 122	-	expression tag
His 123	-	expression tag

P: 529 - 936 (UniProt: P0ABZ6)

PDB	External Database	Details
Gly 487	-	expression tag
Ser 488	-	expression tag
Ser 489	-	expression tag
Ala 490	-	expression tag
Trp 491	-	expression tag
Ser 492	-	expression tag
His 493	-	expression tag
Pro 494	-	expression tag
Gln 495	-	expression tag
Phe 496	-	expression tag
Glu 497	-	expression tag
Lys 498	-	expression tag
Gly 499	-	expression tag
Gly 500	-	expression tag
Gly 501	-	expression tag
Ser 502	-	expression tag
Gly 503	-	expression tag
Gly 504	-	expression tag
Gly 505	-	expression tag
Ser 506	-	expression tag
Gly 507	-	expression tag
Gly 508	-	expression tag
Ser 509	-	expression tag
Ala 510	-	expression tag
Trp 511	-	expression tag
Ser 512	-	expression tag
His 513	-	expression tag
Pro 514	-	expression tag
Gln 515	-	expression tag
Phe 516	-	expression tag
Glu 517	-	expression tag
Lys 518	-	expression tag
Ser 519	-	expression tag
Ser 520	-	expression tag
Gly 521	-	expression tag
Glu 522	-	expression tag
Asn 523	-	expression tag
Leu 524	-	expression tag
Tyr 525	-	expression tag
Phe 526	-	expression tag
Gln 527	-	expression tag
Gly 528	-	expression tag
Cys 535	Lys 27	conflict
Gly 937	-	linker
Gly 938	-	linker

P: 939 - 1300 (UniProt: Q7BSW5)

PDB	External Database	Details
Gly 976	-	insertion
Glu 977	-	insertion
Asn 978	-	insertion
Leu 979	-	insertion
Tyr 980	-	insertion
Phe 981	-	insertion
Gln 982	-	insertion
Gly 983	-	insertion
Gly 984	-	insertion
Cys 1299	Ser 1299	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	289423.4
All*	Total formula weight	289423.4

*Water molecules are not included.