8QOF

Cryo-EM structure of the yeast SPT-Orm2-Dimer complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B, F	Serine palmitoyltransferase 1	polymer	580	64985.5	2	UniProt (P25045) Pfam (PF00155) In PDB	Saccharomyces cerevisiae (baker's yeast)	SPT 1,SPT1,Long chain base biosynthesis protein 1
2	D, H	Serine palmitoyltransferase-regulating protein TSC3	polymer	80	9590.2	2	UniProt (Q3E790) In PDB	Saccharomyces cerevisiae (baker's yeast)	Temperature-sensitive CSG2-mutant suppressor protein 3
3	A, E	ORM2 isoform 1	polymer	216	24830.6	2	UniProt (A0A6L0ZQC3) In PDB	Saccharomyces cerevisiae (baker's yeast)
4	G, C	Serine palmitoyltransferase 2	polymer	561	63189.7	2	UniProt (P40970) Pfam (PF00155) In PDB	Saccharomyces cerevisiae (baker's yeast)	SPT 2,Long chain base biosynthesis protein 2
5	A, E	~{N}-[(2~{S},3~{S},4~{R})-1,3,4-tris(oxidanyl)octadecan-2-yl]heptacosanamide	non-polymer		710.2	2	Chemie (WAR)
6	G, C	PYRIDOXAL-5'-PHOSPHATE	non-polymer		247.1	2	Chemie (PLP)
7	G, C	2-{[(4-O-alpha-D-glucopyranosyl-alpha-D-glucopyranosyl)oxy]methyl}-4-{[(3beta,9beta,14beta,17beta,25R)-spirost-5-en-3-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-alpha-D-glucopyranoside	non-polymer		1165.3	2	Chemie (Q7G)

Sequence modifications

B, F: -21 - 558 (UniProt: P25045)

PDB	External Database	Details
Asp -12	-	insertion
Tyr -11	-	insertion
Lys -10	-	insertion
Asp -9	-	insertion
His -8	-	insertion
Asp -7	-	insertion
Gly -6	-	insertion
Asp -5	-	insertion
Tyr -4	-	insertion
Lys -3	-	insertion
Asp -2	-	insertion
His -1	-	insertion
Asp 0	-	insertion
Ile 1	-	insertion
Asp 2	-	insertion
Tyr 3	-	insertion
Lys 4	-	insertion
Asp 5	-	insertion
Asp 6	-	insertion
Asp 7	-	insertion
Asp 8	-	insertion
Lys 9	-	insertion

A, E: 1 - 216 (UniProt: A0A6L0ZQC3)

PDB	External Database	Details
Ala 46	Ser 46	engineered mutation
Ala 47	Ser 47	engineered mutation
Ala 48	Ser 48	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	325192.1
Non-Polymers*	Number of molecules	6
	Total formula weight	4245.3
All*	Total formula weight	329437.5

*Water molecules are not included.