8QOF
Cryo-EM structure of the yeast SPT-Orm2-Dimer complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (B, F) | Serine palmitoyltransferase 1 | polymer | 580 | 64985.5 | 2 | UniProt (P25045) Pfam (PF00155) | Saccharomyces cerevisiae (baker's yeast) | SPT 1,SPT1,Long chain base biosynthesis protein 1 |
| 2 | C, D (D, H) | Serine palmitoyltransferase-regulating protein TSC3 | polymer | 80 | 9590.2 | 2 | UniProt (Q3E790) | Saccharomyces cerevisiae (baker's yeast) | Temperature-sensitive CSG2-mutant suppressor protein 3 |
| 3 | E, F (A, E) | ORM2 isoform 1 | polymer | 216 | 24830.6 | 2 | UniProt (A0A6L0ZQC3) | Saccharomyces cerevisiae (baker's yeast) | |
| 4 | G, H (G, C) | Serine palmitoyltransferase 2 | polymer | 561 | 63189.7 | 2 | UniProt (P40970) Pfam (PF00155) | Saccharomyces cerevisiae (baker's yeast) | SPT 2,Long chain base biosynthesis protein 2 |
| 5 | I, J (A, E) | ~{N}-[(2~{S},3~{S},4~{R})-1,3,4-tris(oxidanyl)octadecan-2-yl]heptacosanamide | non-polymer | 710.2 | 2 | Chemie (WAR) | |||
| 6 | K, M (G, C) | PYRIDOXAL-5'-PHOSPHATE | non-polymer | 247.1 | 2 | Chemie (PLP) | |||
| 7 | L, N (G, C) | 2-{[(4-O-alpha-D-glucopyranosyl-alpha-D-glucopyranosyl)oxy]methyl}-4-{[(3beta,9beta,14beta,17beta,25R)-spirost-5-en-3-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-alpha-D-glucopyranoside | non-polymer | 1165.3 | 2 | Chemie (Q7G) |
Sequence modifications
B, F: -21 - 558 (UniProt: P25045)
A, E: 1 - 216 (UniProt: A0A6L0ZQC3)
| PDB | External Database | Details |
|---|---|---|
| Asp -12 | - | insertion |
| Tyr -11 | - | insertion |
| Lys -10 | - | insertion |
| Asp -9 | - | insertion |
| His -8 | - | insertion |
| Asp -7 | - | insertion |
| Gly -6 | - | insertion |
| Asp -5 | - | insertion |
| Tyr -4 | - | insertion |
| Lys -3 | - | insertion |
| Asp -2 | - | insertion |
| His -1 | - | insertion |
| Asp 0 | - | insertion |
| Ile 1 | - | insertion |
| Asp 2 | - | insertion |
| Tyr 3 | - | insertion |
| Lys 4 | - | insertion |
| Asp 5 | - | insertion |
| Asp 6 | - | insertion |
| Asp 7 | - | insertion |
| Asp 8 | - | insertion |
| Lys 9 | - | insertion |
| PDB | External Database | Details |
|---|---|---|
| Ala 46 | Ser 46 | engineered mutation |
| Ala 47 | Ser 47 | engineered mutation |
| Ala 48 | Ser 48 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 325192.1 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 4245.3 | |
| All* | Total formula weight | 329437.5 |
