8QOA

Structure of SecM-stalled Escherichia coli 70S ribosome

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	0	Large ribosomal subunit protein bL33	polymer	55	6388.6	1	UniProt (P0A7N9) Pfam (PF00471) In PDB	Escherichia coli BW25113	50S ribosomal protein L33
2	1	Large ribosomal subunit protein bL34	polymer	46	5397.5	1	UniProt (P0A7P5) Pfam (PF00468) In PDB	Escherichia coli BW25113	50S ribosomal protein L34
3	2	Large ribosomal subunit protein bL35	polymer	65	7313.0	1	UniProt (P0A7Q1) Pfam (PF01632) In PDB	Escherichia coli BW25113	50S ribosomal protein L35,Ribosomal protein A
4	3	Large ribosomal subunit protein bL36A	polymer	38	4377.4	1	UniProt (P0A7Q6) Pfam (PF00444) In PDB	Escherichia coli BW25113	50S ribosomal protein L36,Ribosomal protein B
5	5	E-site tRNA	polymer	2	589.4	1		Escherichia coli BW25113
6	B	30S ribosomal protein S2	polymer	241	26781.7	1	UniProt (P0A7V0) Pfam (PF00318) In PDB	Escherichia coli BW25113	Small ribosomal subunit protein uS2
7	C	Small ribosomal subunit protein uS3	polymer	233	26031.3	1	UniProt (P0A7V3) Pfam (PF07650) Pfam (PF00189) In PDB	Escherichia coli BW25113	30S ribosomal protein S3
8	D	Small ribosomal subunit protein uS4	polymer	206	23514.2	1	UniProt (P0A7V8) Pfam (PF01479) Pfam (PF00163) In PDB	Escherichia coli BW25113	30S ribosomal protein S4
9	E	Small ribosomal subunit protein uS5	polymer	167	17629.4	1	UniProt (P0A7W1) Pfam (PF00333) Pfam (PF03719) In PDB	Escherichia coli BW25113	30S ribosomal protein S5
10	F	30S ribosomal protein S6, fully modified isoform	polymer	135	15727.5	1	UniProt (P02358) Pfam (PF01250) In PDB	Escherichia coli BW25113
11	G	30S ribosomal protein S7	polymer	179	20055.2	1	UniProt (P02359) Pfam (PF00177) In PDB	Escherichia coli BW25113	Small ribosomal subunit protein uS7
12	H	Small ribosomal subunit protein uS8	polymer	130	14146.6	1	UniProt (P0A7W7) Pfam (PF00410) In PDB	Escherichia coli BW25113	30S ribosomal protein S8
13	I	Small ribosomal subunit protein uS9	polymer	130	14886.3	1	UniProt (P0A7X3) Pfam (PF00380) In PDB	Escherichia coli BW25113	30S ribosomal protein S9
14	L	Small ribosomal subunit protein uS12	polymer	124	13814.2	1	UniProt (P0A7S3) Pfam (PF00164) In PDB	Escherichia coli BW25113	30S ribosomal protein S12
15	M	Small ribosomal subunit protein uS13	polymer	118	13128.5	1	UniProt (P0A7S9) Pfam (PF00416) In PDB	Escherichia coli BW25113	30S ribosomal protein S13
16	N	Small ribosomal subunit protein uS14	polymer	101	11606.6	1	UniProt (P0AG59) Pfam (PF00253) In PDB	Escherichia coli BW25113	30S ribosomal protein S14
17	O	Small ribosomal subunit protein uS15	polymer	89	10290.8	1	UniProt (P0ADZ4) Pfam (PF00312) In PDB	Escherichia coli BW25113	30S ribosomal protein S15
18	P	30S ribosomal protein S16	polymer	82	9207.6	1	UniProt (P0A7T3) Pfam (PF00886) In PDB	Escherichia coli BW25113	Small ribosomal subunit protein bS16
19	Q	Small ribosomal subunit protein uS17	polymer	84	9724.5	1	UniProt (P0AG63) Pfam (PF00366) In PDB	Escherichia coli BW25113	30S ribosomal protein S17
20	R	Small ribosomal subunit protein bS18	polymer	75	9005.5	1	UniProt (P0A7T7) Pfam (PF01084) In PDB	Escherichia coli BW25113	30S ribosomal protein S18
21	S	Small ribosomal subunit protein uS19	polymer	92	10455.4	1	UniProt (P0A7U3) Pfam (PF00203) In PDB	Escherichia coli BW25113	30S ribosomal protein S19
22	T	30S ribosomal protein S20	polymer	87	9708.5	1	UniProt (P0A7U7) Pfam (PF01649) In PDB	Escherichia coli BW25113	Small ribosomal subunit protein bS20
23	U	Small ribosomal subunit protein bS21	polymer	71	8524.0	1	UniProt (P68679) Pfam (PF01165) In PDB	Escherichia coli BW25113	30S ribosomal protein S21
24	X	mRNA	polymer	9	2823.7	1		Escherichia coli BW25113
25	Z	P-site tRNA-gly	polymer	76	24478.5	1		Escherichia coli BW25113
26	b	5S rRNA	polymer	120	38790.1	1	GenBank (1845258627)	Escherichia coli BW25113
27	c	Large ribosomal subunit protein uL2	polymer	273	29923.6	1	UniProt (P60422) Pfam (PF00181) Pfam (PF03947) In PDB	Escherichia coli BW25113	50S ribosomal protein L2
28	d	Large ribosomal subunit protein uL3	polymer	209	22291.6	1	UniProt (P60438) Pfam (PF00297) In PDB	Escherichia coli BW25113	50S ribosomal protein L3
29	e	Large ribosomal subunit protein uL4	polymer	201	22121.6	1	UniProt (P60723) Pfam (PF00573) In PDB	Escherichia coli BW25113	50S ribosomal protein L4
30	f	Large ribosomal subunit protein uL5	polymer	179	20333.6	1	UniProt (P62399) Pfam (PF00281) Pfam (PF00673) In PDB	Escherichia coli BW25113	50S ribosomal protein L5
31	g	Large ribosomal subunit protein uL6	polymer	177	18932.8	1	UniProt (P0AG55) Pfam (PF00347) In PDB	Escherichia coli BW25113	50S ribosomal protein L6
32	h	Large ribosomal subunit protein bL9	polymer	149	15789.0	1	UniProt (P0A7R1) Pfam (PF01281) Pfam (PF03948) In PDB	Escherichia coli BW25113	50S ribosomal protein L9
33	i	Large ribosomal subunit protein uL13	polymer	142	16050.6	1	UniProt (P0AA10) Pfam (PF00572) In PDB	Escherichia coli BW25113	50S ribosomal protein L13
34	j	Large ribosomal subunit protein uL14	polymer	123	13565.1	1	UniProt (P0ADY3) Pfam (PF00238) In PDB	Escherichia coli BW25113	50S ribosomal protein L14
35	k	50S ribosomal protein L15	polymer	144	15008.5	1	UniProt (P02413) Pfam (PF00828) In PDB	Escherichia coli BW25113	Large ribosomal subunit protein uL15
36	m	Large ribosomal subunit protein bL17	polymer	127	14393.7	1	UniProt (P0AG44) Pfam (PF01196) In PDB	Escherichia coli BW25113	50S ribosomal protein L17
37	n	Large ribosomal subunit protein uL18	polymer	117	12794.7	1	UniProt (P0C018) Pfam (PF00861) In PDB	Escherichia coli BW25113	50S ribosomal protein L18
38	o	Large ribosomal subunit protein bL19	polymer	115	13159.3	1	UniProt (P0A7K6) Pfam (PF01245) In PDB	Escherichia coli BW25113	50S ribosomal protein L19
39	p	Large ribosomal subunit protein bL20	polymer	118	13528.0	1	UniProt (P0A7L3) Pfam (PF00453) In PDB	Escherichia coli BW25113	50S ribosomal protein L20
40	q	Large ribosomal subunit protein bL21	polymer	103	11586.4	1	UniProt (P0AG48) Pfam (PF00829) In PDB	Escherichia coli BW25113	50S ribosomal protein L21
41	r	Large ribosomal subunit protein uL22	polymer	110	12253.4	1	UniProt (P61175) Pfam (PF00237) In PDB	Escherichia coli BW25113	50S ribosomal protein L22
42	s	Large ribosomal subunit protein uL23	polymer	100	11222.2	1	UniProt (P0ADZ0) Pfam (PF00276) In PDB	Escherichia coli BW25113	50S ribosomal protein L23
43	t	Large ribosomal subunit protein uL24	polymer	104	11339.3	1	UniProt (P60624) Pfam (PF00467) Pfam (PF17136) In PDB	Escherichia coli BW25113	50S ribosomal protein L24
44	u	50S ribosomal protein L25	polymer	94	10713.5	1	UniProt (P68919) Pfam (PF01386) In PDB	Escherichia coli BW25113	Large ribosomal subunit protein bL25
45	v	Large ribosomal subunit protein bL27	polymer	85	9146.5	1	UniProt (P0A7L8) Pfam (PF01016) In PDB	Escherichia coli BW25113	50S ribosomal protein L27
46	w	Large ribosomal subunit protein bL28	polymer	78	9027.6	1	UniProt (P0A7M2) Pfam (PF00830) In PDB	Escherichia coli BW25113	50S ribosomal protein L28
47	x	Large ribosomal subunit protein uL29	polymer	63	7286.5	1	UniProt (P0A7M6) Pfam (PF00831) In PDB	Escherichia coli BW25113	50S ribosomal protein L29
48	y	Large ribosomal subunit protein uL30	polymer	59	6554.8	1	UniProt (P0AG51) Pfam (PF00327) In PDB	Escherichia coli BW25113	50S ribosomal protein L30
49	z	Large ribosomal subunit protein bL32	polymer	57	6463.4	1	UniProt (P0A7N4) Pfam (PF01783) In PDB	Escherichia coli BW25113	50S ribosomal protein L32
50	Y	A-site tRNA-pro	polymer	77	24862.7	1	GenBank (1848934315)	Escherichia coli BW25113
51	4	Large ribosomal subunit protein bL31A	polymer	70	7887.1	1	UniProt (P0A7M9) Pfam (PF01197) In PDB	Escherichia coli BW25113	50S ribosomal protein L31
52	a	23S rRNA	polymer	2904	941811.6	1		Escherichia coli BW25113
53	A	16S rRNA	polymer	1542	499761.2	1	GenBank (1789840096)	Escherichia coli BW25113
54	J	Small ribosomal subunit protein uS10	polymer	103	11755.6	1	UniProt (P0A7R5) Pfam (PF00338) In PDB	Escherichia coli BW25113	30S ribosomal protein S10,Transcription termination/antitermination protein NusE
55	6	Secretion monitor	polymer	34	3813.3	1	UniProt (P62395) In PDB	Escherichia coli BW25113
56	l	Large ribosomal subunit protein uL16	polymer	136	15329.3	1	UniProt (P0ADY7) Pfam (PF00252) In PDB	Escherichia coli BW25113	50S ribosomal protein L16
57	K	Small ribosomal subunit protein uS11	polymer	129	13872.0	1	UniProt (P0A7R9) Pfam (PF00411) In PDB	Escherichia coli BW25113	30S ribosomal protein S11
58	4, 3	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
59	a, A, D, F, M...	POTASSIUM ION	non-polymer		39.1	132	Chemie (K)
60	z, a, A, X, Z...	MAGNESIUM ION	non-polymer		24.3	288	Chemie (MG)
61	Y	PROLINE	non-polymer		115.1	1	Chemie (PRO)
62		water	water		18.0	5753	Chemie (HOH)

Sequence modifications

l: 1 - 136 (UniProt: P0ADY7)

PDB	External Database	Details
Ms6 82	Met 82	variant

K: 1 - 129 (UniProt: P0A7R9)

PDB	External Database	Details
Ias 119	Asn 119	variant

Contents of the asymmetric unit

Polymers	Number of chains	57
	Total formula weight	2196973.9
Non-Polymers*	Number of molecules	423
	Total formula weight	12406.7
All*	Total formula weight	2209380.6

*Water molecules are not included.