8QIZ
Crystal structure of Paradendryphiella salina PL7C alginate lyase mutant H124N soaked with with penta-mannuronic acid
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Alginate lyase | polymer | 234 | 25520.2 | 1 | UniProt (A0A7I9C8Z1) Pfam (PF08787) | Paradendryphiella salina | |
| 2 | B (B) | beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-alpha-L-gulopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid | branched | 1074.8 | 1 | In PDB | |||
| 3 | C (C) | beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid | branched | 722.5 | 1 | In PDB GlyTouCan (G72539QT) | |||
| 4 | D (A) | water | water | 18.0 | 337 | Chemie (HOH) |
Sequence modifications
A: 17 - 240 (UniProt: A0A7I9C8Z1)
| PDB | External Database | Details |
|---|---|---|
| Glu 15 | - | expression tag |
| Phe 16 | - | expression tag |
| Asn 124 | His 124 | engineered mutation |
| Val 241 | - | expression tag |
| Asp 242 | - | expression tag |
| His 243 | - | expression tag |
| His 244 | - | expression tag |
| His 245 | - | expression tag |
| His 246 | - | expression tag |
| His 247 | - | expression tag |
| His 248 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 25520.2 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 1797.3 | |
| All* | Total formula weight | 27317.5 |
