8QHC

Cryo-EM structure of SidH from Legionella pneumophila in complex with LubX

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	C	Elongation factor Tu	polymer	394	43370.5	1	UniProt (E2QJ06) Pfam (PF00009) Pfam (PF03144) Pfam (PF03143) UniProt (by SIFTS) (P0CE48) In PDB	Escherichia coli 'BL21-Gold(DE3)pLysS AG'
2	B	t-RNA	polymer	76	24620.8	1		Escherichia coli 'BL21-Gold(DE3)pLysS AG'
3	A	Protein SidH	polymer	2244	255113.7	1	UniProt (Q6RCQ4) In PDB	Legionella pneumophila
4	D	E3 ubiquitin--protein ligase	polymer	264	30506.8	1	UniProt (A0A2S6F0E3) Pfam (PF04564) UniProt (by SIFTS) (Q5ZRQ0) In PDB	Legionella pneumophila
5	C	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
6	C	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)

Sequence modifications

A: 1 - 2225 (UniProt: Q6RCQ4)

PDB	External Database	Details
Met -18	-	initiating methionine
Gly -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Leu -5	-	expression tag
Val -4	-	expression tag
Pro -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

D: 1 - 246 (UniProt: A0A2S6F0E3)

PDB	External Database	Details
Met -17	-	initiating methionine
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ala -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Leu -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Pro 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	353611.8
Non-Polymers*	Number of molecules	2
	Total formula weight	547.5
All*	Total formula weight	354159.3

*Water molecules are not included.