8QG0

Archaeoglobus fulgidus AfAgo complex with AfAgo-N protein (fAfAgo) bound with 17 nt RNA guide and 17 nt DNA target

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Piwi protein	polymer	427	49302.4	1	UniProt (A0A101DYI0) Pfam (PF02171) UniProt (by SIFTS) (O28951)	Archaeoglobus fulgidus
2	B (B)	AfAgo-N protein	polymer	273	31356.6	1	UniProt (A0A075WKW4)	Archaeoglobus fulgidus
3	C (P)	DNA target 17 nt	polymer	17	5202.4	1		Escherichia coli
4	D (Q)	RNA guide 17 nt	polymer	17	5427.3	1		Escherichia coli

Sequence modifications

B: 2 - 250 (UniProt: A0A075WKW4)

PDB	External Database	Details
Met -22	-	initiating methionine
Gly -21	-	expression tag
Gly -20	-	expression tag
Ser -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Gly -12	-	expression tag
Met -11	-	expression tag
Ala -10	-	expression tag
Ser -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Gly 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	91288.7
All*	Total formula weight	91288.7

*Water molecules are not included.