8QFU

Diels-Alderase AbyU mutant - Y76F

Replaces:  7Z6P

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	YD repeat-containing protein	polymer	160	17747.9	4	UniProt (F4F7G1)	Micromonospora maris AB-18-032
2	E, F, G, H (A, B, C, D)	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	4	Chemie (EPE)
3	I, J, K, L (A, B, C, D)	water	water		18.0	484	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 141 (UniProt: F4F7G1)

PDB	External Database	Details
Met -18	-	initiating methionine
Ala -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Leu -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Pro 0	-	expression tag
Phe 76	Tyr 76	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	70991.6
Non-Polymers*	Number of molecules	4
	Total formula weight	953.2
All*	Total formula weight	71944.8

*Water molecules are not included.