8QFS

Cryo-EM structure of SidH from Legionella pneumophila

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	C	Elongation factor Tu	polymer	394	43370.5	1	UniProt (E2QJ06) Pfam (PF00009) Pfam (PF03144) Pfam (PF03143) UniProt (by SIFTS) (P0CE48) In PDB	Escherichia coli 'BL21-Gold(DE3)pLysS AG'
2	B	tRNA	polymer	76	24620.8	1		Escherichia coli 'BL21-Gold(DE3)pLysS AG'
3	A	Protein SidH	polymer	2248	255821.6	1	UniProt (Q6RCQ4) UniProt (by SIFTS) (Q5ZRQ1) In PDB	Escherichia coli 'BL21-Gold(DE3)pLysS AG'
4	C	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5	C	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)

Sequence modifications

A: 1 - 2225 (UniProt: Q6RCQ4)

PDB	External Database	Details
Met -22	-	initiating methionine
Lys -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ala -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Leu -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Pro 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	323812.8
Non-Polymers*	Number of molecules	2
	Total formula weight	547.5
All*	Total formula weight	324360.3

*Water molecules are not included.