8QEO

cryo-EM structure complex of Frizzled-7 and Clostridioides difficile toxin B

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Toxin B	polymer	2397	273551.6	1	UniProt (P18177) Pfam (PF12918) Pfam (PF12919) Pfam (PF11713) Pfam (PF12920) Pfam (PF01473) Pfam (PF19127)	Clostridioides difficile
2	B (B)	Frizzled-7	polymer	603	67460.9	1	UniProt (O75084) Pfam (PF01392) Pfam (PF01534)	Homo sapiens (human)	Fz-7,hFz7,FzE3
3	C (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

A: 1 - 2366 (UniProt: P18177)

PDB	External Database	Details
Met -13	-	initiating methionine
Asp -12	-	expression tag
Lys -11	-	expression tag
Leu -10	-	expression tag
Val -9	-	expression tag
His -8	-	expression tag
Leu -7	-	expression tag
Asn -6	-	expression tag
Gln -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Lys -2	-	expression tag
Cys -1	-	expression tag
Thr 0	-	expression tag
Gly 2367	-	expression tag
Tyr 2368	-	expression tag
Arg 2369	-	expression tag
Pro 2370	-	expression tag
His 2371	-	expression tag
Ala 2372	-	expression tag
Gly 2373	-	expression tag
Leu 2374	-	expression tag
Arg 2375	-	expression tag
Gly 2376	-	expression tag
Ser 2377	-	expression tag
His 2378	-	expression tag
His 2379	-	expression tag
His 2380	-	expression tag
His 2381	-	expression tag
His 2382	-	expression tag
His 2383	-	expression tag

B: 33 - 574 (UniProt: O75084)

PDB	External Database	Details
Met 10	-	initiating methionine
Lys 11	-	expression tag
Thr 12	-	expression tag
Ile 13	-	expression tag
Ile 14	-	expression tag
Ala 15	-	expression tag
Leu 16	-	expression tag
Ser 17	-	expression tag
Tyr 18	-	expression tag
Ile 19	-	expression tag
Phe 20	-	expression tag
Cys 21	-	expression tag
Leu 22	-	expression tag
Val 23	-	expression tag
Phe 24	-	expression tag
Ala 25	-	expression tag
Asp 26	-	expression tag
Tyr 27	-	expression tag
Lys 28	-	expression tag
Asp 29	-	expression tag
Asp 30	-	expression tag
Asp 31	-	expression tag
Asp 32	-	expression tag
Ser 575	-	expression tag
Arg 576	-	expression tag
Leu 577	-	expression tag
Glu 578	-	expression tag
Val 579	-	expression tag
Leu 580	-	expression tag
Phe 581	-	expression tag
Gln 582	-	expression tag
Gly 583	-	expression tag
Pro 584	-	expression tag
Trp 585	-	expression tag
Ser 586	-	expression tag
His 587	-	expression tag
Pro 588	-	expression tag
Gln 589	-	expression tag
Phe 590	-	expression tag
Glu 591	-	expression tag
Lys 592	-	expression tag
Gly 593	-	expression tag
Gly 594	-	expression tag
Gly 595	-	expression tag
Ser 596	-	expression tag
Gly 597	-	expression tag
Gly 598	-	expression tag
Gly 599	-	expression tag
Ser 600	-	expression tag
Gly 601	-	expression tag
Gly 602	-	expression tag
Gly 603	-	expression tag
Ser 604	-	expression tag
Trp 605	-	expression tag
Ser 606	-	expression tag
His 607	-	expression tag
Pro 608	-	expression tag
Gln 609	-	expression tag
Phe 610	-	expression tag
Glu 611	-	expression tag
Lys 612	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	341012.4
Non-Polymers*	Number of molecules	1
	Total formula weight	65.4
All*	Total formula weight	341077.8

*Water molecules are not included.