8QDD

Structure of mBaoJin at pH 8.5

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, C)	mBaoJin	polymer	236	26841.1	2	UniProt (A0A8S0GSD4) Pfam (PF01353)	Cytaeis uchidae	Fluorescent protein,mBaoJin
2	C, E (A, C)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
3	D, F (A, C)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
4	G, H (A, C)	water	water		18.0	791	Chemie (HOH)

Sequence modifications

A, C: 1 - 215 (UniProt: A0A8S0GSD4)

PDB	External Database	Details
Arg -10	-	expression tag
Ser -9	-	expression tag
Met -8	-	expression tag
Val -7	-	expression tag
Ser -6	-	expression tag
Lys -5	-	expression tag
Gly -4	-	expression tag
Glu -3	-	expression tag
Glu -2	-	expression tag
Glu -1	-	expression tag
Asn 0	-	expression tag
Thr 55	Ser 55	engineered mutation
Cr2 57	Gly 57	chromophore
Cr2 57	Tyr 58	chromophore
Cr2 57	Gly 59	chromophore
Arg 73	His 75	engineered mutation
Gly 78	Glu 80	engineered mutation
Pro 138	Gln 140	engineered mutation
Gln 139	His 141	engineered mutation
Tyr 163	Cys 165	engineered mutation
Ala 166	Val 168	engineered mutation
Tyr 169	Asn 171	engineered mutation
Ala 199	Thr 201	engineered mutation
Lys 216	-	expression tag
Gly 217	-	expression tag
Met 218	-	expression tag
Asp 219	-	expression tag
Glu 220	-	expression tag
Leu 221	-	expression tag
Tyr 222	-	expression tag
Lys 223	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	53682.2
Non-Polymers*	Number of molecules	4
	Total formula weight	263.0
All*	Total formula weight	53945.3

*Water molecules are not included.