8Q63

Cryo-EM structure of IC8', a second state of yeast mitochondrial RNA polymerase transcription initiation complex with 8-mer RNA, pppGpGpUpApApApUpG

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Mitochondrial transcription factor 1	polymer	354	41151.2	1	UniProt (P14908) Pfam (PF00398)	Saccharomyces cerevisiae S288C	Mitochondrial transcription factor mtTFB,Mitochondrial-specificity factor,RF1023
2	B (A)	DNA-directed RNA polymerase, mitochondrial	polymer	1262	143282.7	1	UniProt (P13433) Pfam (PF14700) Pfam (PF00940)	Saccharomyces cerevisiae S288C
3	C (N)	Non-template DNA (37-mer)	polymer	37	11468.5	1		Saccharomyces cerevisiae S288C
4	D (T)	Template DNA (37-MER)	polymer	37	11298.3	1		Saccharomyces cerevisiae S288C
5	E (C)	RNA (pppGpGpUpApApApUpG)	polymer	7	2245.4	1		Saccharomyces cerevisiae S288C
6	F (C)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)

Sequence modifications

B: 2 - 341 (UniProt: P14908)

PDB	External Database	Details
Met -12	-	initiating methionine
Gly -11	-	expression tag
Gly -10	-	expression tag
Ser -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Met -1	-	expression tag
Ala 0	-	expression tag
Ser 1	-	expression tag

A: 100 - 1351 (UniProt: P13433)

PDB	External Database	Details
Gly 90	-	expression tag
Ala 91	-	expression tag
Met 92	-	expression tag
Gly 93	-	expression tag
Ser 94	-	expression tag
Gly 95	-	expression tag
Ile 96	-	expression tag
Gln 97	-	expression tag
Arg 98	-	expression tag
Pro 99	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	209446.0
Non-Polymers*	Number of molecules	1
	Total formula weight	523.2
All*	Total formula weight	209969.2

*Water molecules are not included.