8Q63
Cryo-EM structure of IC8', a second state of yeast mitochondrial RNA polymerase transcription initiation complex with 8-mer RNA, pppGpGpUpApApApUpG
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | Mitochondrial transcription factor 1 | polymer | 354 | 41151.2 | 1 | UniProt (P14908) Pfam (PF00398) | Saccharomyces cerevisiae S288C | Mitochondrial transcription factor mtTFB,Mitochondrial-specificity factor,RF1023 |
| 2 | B (A) | DNA-directed RNA polymerase, mitochondrial | polymer | 1262 | 143282.7 | 1 | UniProt (P13433) Pfam (PF14700) Pfam (PF00940) | Saccharomyces cerevisiae S288C | |
| 3 | C (N) | Non-template DNA (37-mer) | polymer | 37 | 11468.5 | 1 | Saccharomyces cerevisiae S288C | ||
| 4 | D (T) | Template DNA (37-MER) | polymer | 37 | 11298.3 | 1 | Saccharomyces cerevisiae S288C | ||
| 5 | E (C) | RNA (pppGpGpUpApApApUpG) | polymer | 7 | 2245.4 | 1 | Saccharomyces cerevisiae S288C | ||
| 6 | F (C) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) |
Sequence modifications
B: 2 - 341 (UniProt: P14908)
A: 100 - 1351 (UniProt: P13433)
| PDB | External Database | Details |
|---|---|---|
| Met -12 | - | initiating methionine |
| Gly -11 | - | expression tag |
| Gly -10 | - | expression tag |
| Ser -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Met -1 | - | expression tag |
| Ala 0 | - | expression tag |
| Ser 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 90 | - | expression tag |
| Ala 91 | - | expression tag |
| Met 92 | - | expression tag |
| Gly 93 | - | expression tag |
| Ser 94 | - | expression tag |
| Gly 95 | - | expression tag |
| Ile 96 | - | expression tag |
| Gln 97 | - | expression tag |
| Arg 98 | - | expression tag |
| Pro 99 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 209446.0 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 523.2 | |
| All* | Total formula weight | 209969.2 |
