8Q2N

Cas1-Cas2 CRISPR integrase bound to prespacer and target DNA, Streptococcus thermophilus DGCC 7710 CRISPR3 system

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D	CRISPR-associated endoribonuclease Cas2	polymer	114	13431.6	2	UniProt (G3ECR3) Pfam (PF09827) In PDB	Streptococcus thermophilus DGCC 7710
2	B, C, E, F	CRISPR-associated endonuclease Cas1	polymer	302	35363.5	4	UniProt (G3ECR2) Pfam (PF01867) In PDB	Streptococcus thermophilus DGCC 7710
3	G	Prespacer DNA, chain G	polymer	26	8062.3	1		synthetic construct
4	H	Prespacer DNA, chain H	polymer	26	7917.1	1		synthetic construct
5	I	Integration target, chain I	polymer	48	14729.5	1		synthetic construct
6	J	Integration target, chain J	polymer	48	14835.5	1		synthetic construct
7	A, D	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

B, C, E, F: 1 - 289 (UniProt: G3ECR2)

PDB	External Database	Details
Leu 290	-	expression tag
Glu 291	-	expression tag
Ser 292	-	expression tag
Gly 293	-	expression tag
Trp 294	-	expression tag
Ser 295	-	expression tag
His 296	-	expression tag
Pro 297	-	expression tag
Gln 298	-	expression tag
Phe 299	-	expression tag
Glu 300	-	expression tag
Lys 301	-	expression tag
Ala 302	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	213861.3
Non-Polymers*	Number of molecules	2
	Total formula weight	48.6
All*	Total formula weight	213909.9

*Water molecules are not included.