8Q2N
Cas1-Cas2 CRISPR integrase bound to prespacer and target DNA, Streptococcus thermophilus DGCC 7710 CRISPR3 system
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D (A, D) | CRISPR-associated endoribonuclease Cas2 | polymer | 114 | 13431.6 | 2 | UniProt (G3ECR3) Pfam (PF09827) | Streptococcus thermophilus DGCC 7710 | |
| 2 | B, C, E, F (B, C, E, F) | CRISPR-associated endonuclease Cas1 | polymer | 302 | 35363.5 | 4 | UniProt (G3ECR2) Pfam (PF01867) | Streptococcus thermophilus DGCC 7710 | |
| 3 | G (G) | Prespacer DNA, chain G | polymer | 26 | 8062.3 | 1 | synthetic construct | ||
| 4 | H (H) | Prespacer DNA, chain H | polymer | 26 | 7917.1 | 1 | synthetic construct | ||
| 5 | I (I) | Integration target, chain I | polymer | 48 | 14729.5 | 1 | synthetic construct | ||
| 6 | J (J) | Integration target, chain J | polymer | 48 | 14835.5 | 1 | synthetic construct | ||
| 7 | K, L (A, D) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) |
Sequence modifications
B, C, E, F: 1 - 289 (UniProt: G3ECR2)
| PDB | External Database | Details |
|---|---|---|
| Leu 290 | - | expression tag |
| Glu 291 | - | expression tag |
| Ser 292 | - | expression tag |
| Gly 293 | - | expression tag |
| Trp 294 | - | expression tag |
| Ser 295 | - | expression tag |
| His 296 | - | expression tag |
| Pro 297 | - | expression tag |
| Gln 298 | - | expression tag |
| Phe 299 | - | expression tag |
| Glu 300 | - | expression tag |
| Lys 301 | - | expression tag |
| Ala 302 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 213861.3 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 48.6 | |
| All* | Total formula weight | 213909.9 |
