8PVY

Cryo-EM structure of the human BRISC dimer complex bound to compound FX-171-C

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, G, I (A, C, G, I)	Lys-63-specific deubiquitinase BRCC36	polymer	312	35703.5	4	UniProt (P46736) Pfam (PF01398) Pfam (PF18110)	Homo sapiens (human)	BRCA1-A complex subunit BRCC36,BRCA1/BRCA2-containing complex subunit 3,BRCA1/BRCA2-containing complex subunit 36,BRISC complex subunit BRCC36
2	B, D, H, J (B, D, H, J)	BRISC complex subunit Abraxas 2	polymer	267	31033.9	4	UniProt (Q15018) Pfam (PF21125)	Homo sapiens (human)	Abraxas brother protein 1,Protein FAM175B
3	E, F, K, L (E, F, K, L)	BRISC and BRCA1-A complex member 2	polymer	385	43721.6	4	UniProt (Q9NXR7) Pfam (PF06113)	Homo sapiens (human)	BRCA1-A complex subunit BRE,BRCA1/BRCA2-containing complex subunit 45,Brain and reproductive organ-expressed protein
4	M, N, O, P (M, N, O, P)	BRISC and BRCA1-A complex member 1	polymer	259	29439.7	4	UniProt (Q9NWV8)	Homo sapiens (human)	Mediator of RAP80 interactions and targeting subunit of 40 kDa,New component of the BRCA1-A complex
5	Q, R, T, U (A, C, G, I)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
6	S, V (D, I)	N-[(1R,5S)-3-azabicyclo[3.1.0]hexan-6-yl]-1-[2,6-bis(chloranyl)phenyl]carbonyl-4-[[2,6-bis(chloranyl)phenyl]carbonylamino]pyrazole-3-carboxamide	non-polymer		553.2	2	Chemie (G1V)

Sequence modifications

E, F, K, L: 1 - 383 (UniProt: Q9NXR7)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 0	-	expression tag

M, N, O, P: 72 - 329 (UniProt: Q9NWV8)

PDB	External Database	Details
Met 71	-	initiating methionine

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	559595.0
Non-Polymers*	Number of molecules	6
	Total formula weight	1368.1
All*	Total formula weight	560963.1

*Water molecules are not included.