8PVV

Archaeoglobus fulgidus AfAgo complex with AfAgo-N protein (fAfAgo) bound with 30 nt RNA guide and 51 nt DNA target

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Piwi protein	polymer	427	49302.4	1	UniProt (A0A101DYI0) Pfam (PF02171) UniProt (by SIFTS) (O28951) In PDB	Archaeoglobus fulgidus
2	C	Archaeoglobus fulgidus AfAgo-N protein	polymer	273	31356.6	1	UniProt (A0A075WKW4) In PDB	Archaeoglobus fulgidus DSM 8774
3	R	RNA (30-MER)	polymer	30	9793.0	1		Escherichia coli
4	S	DNA (51-MER)	polymer	51	15579.9	1		Escherichia coli
5	R	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
6		water	water		18.0	36	Chemie (HOH)

Sequence modifications

C: 2 - 250 (UniProt: A0A075WKW4)

PDB	External Database	Details
Met -22	-	initiating methionine
Gly -21	-	expression tag
Gly -20	-	expression tag
Ser -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Gly -12	-	expression tag
Met -11	-	expression tag
Ala -10	-	expression tag
Ser -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Gly 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	106031.9
Non-Polymers*	Number of molecules	1
	Total formula weight	24.3
All*	Total formula weight	106056.2

*Water molecules are not included.