8PVM
formaldehyde-inhibited [FeFe]-hydrogenase CpI from Clostridium pasteurianum, variant C299D
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | Iron hydrogenase 1 | polymer | 584 | 65123.4 | 2 | UniProt (P29166) Pfam (PF13510) Pfam (PF12838) Pfam (PF02906) Pfam (PF02256) In PDB | Clostridium pasteurianum | CpI,Fe-only hydrogenase,[Fe] hydrogenase |
| 2 | A, B | dicarbonyl[bis(cyanide-kappaC)]-mu-(iminodimethanethiolatato-1kappaS:2kappaS)-mu-(oxomethylidene)diiron(2+) | non-polymer | 355.0 | 2 | Chemie (402) | |||
| 3 | A, B | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 8 | Chemie (SF4) | |||
| 4 | A, B | FE2/S2 (INORGANIC) CLUSTER | non-polymer | 175.8 | 2 | Chemie (FES) | |||
| 5 | A, B | methyl radical | non-polymer | 15.0 | 3 | Chemie (74C) | |||
| 6 | B, A | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
| 7 | B, A | FORMYL GROUP | non-polymer | 30.0 | 2 | Chemie (FOR) | |||
| 8 | B, A | MAGNESIUM ION | non-polymer | 24.3 | 5 | Chemie (MG) | |||
| 9 | B, A | CHLORIDE ION | non-polymer | 35.5 | 6 | Chemie (CL) | |||
| 10 | water | water | 18.0 | 1158 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 574 (UniProt: P29166)
| PDB | External Database | Details |
|---|---|---|
| Asp 299 | Cys 299 | conflict |
| Ser 575 | - | expression tag |
| Ala 576 | - | expression tag |
| Trp 577 | - | expression tag |
| Ser 578 | - | expression tag |
| His 579 | - | expression tag |
| Pro 580 | - | expression tag |
| Gln 581 | - | expression tag |
| Phe 582 | - | expression tag |
| Glu 583 | - | expression tag |
| Lys 584 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 130246.7 | |
| Non-Polymers* | Number of molecules | 32 |
| Total formula weight | 4682.4 | |
| All* | Total formula weight | 134929.2 |
