8PUN

Old Yellow Enzyme from the thermophilic Ferrovum sp. JA12

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	NADPH dehydrogenase	polymer	373	40756.2	4	UniProt (A0A0R0LNE8) Pfam (PF00724) In PDB	Ferrovum sp. JA12
2	A, B, C, D	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	4	Chemie (FMN)
3	A, C	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	3	Chemie (PEG)
4	A, B	2-ethyl-2-(hydroxymethyl)propane-1,3-diol	non-polymer		134.2	2	Chemie (9D2)
5	D, A, B, C	YTTRIUM (III) ION	non-polymer		88.9	7	Chemie (YT3)
6	D, A, B, C	CHLORIDE ION	non-polymer		35.5	5	Chemie (CL)
7	B	TETRAETHYLENE GLYCOL	non-polymer		194.2	1	Chemie (PG4)
8	B	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
9	D, C	1,2-ETHANEDIOL	non-polymer		62.1	4	Chemie (EDO)
10		water	water		18.0	642	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 354 (UniProt: A0A0R0LNE8)

PDB	External Database	Details
Met -18	-	initiating methionine
Gly -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Leu -5	-	expression tag
Val -4	-	expression tag
Pro -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	163024.9
Non-Polymers*	Number of molecules	27
	Total formula weight	3804.4
All*	Total formula weight	166829.3

*Water molecules are not included.