8PT2

Tilapia Lake Virus polymerase in vRNA pre-initiation state mode B (transcriptase conformation)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Polymerase acidic protein (PA-like)	polymer	419	47780.5	1	UniProt (A0A142I7Z3)	Tilapia lake virus
2	B (B)	Putative PB1	polymer	519	57179.4	1	UniProt (A0A1Y9SHW4)	Tilapia lake virus
3	C (C)	RNA-dependent RNA polymerase	polymer	478	53782.1	1	UniProt (A0A7G3S745)	Tilapia lake virus
4	D, E (V, D)	5' vRNA end - vRNA loop (40-mer)	polymer	40	12736.5	2		Tilapia lake virus
5	F, G, H (A, C)	ZINC ION	non-polymer		65.4	3	Chemie (ZN)
6	I (B)	water	water		18.0	1	Chemie (HOH)

Sequence modifications

C: 1 - 457 (UniProt: A0A7G3S745)

PDB	External Database	Details
Lys 391	Arg 391	conflict
Gly 458	-	expression tag
Ser 459	-	expression tag
Gly 460	-	expression tag
Ser 461	-	expression tag
Glu 462	-	expression tag
Asn 463	-	expression tag
Leu 464	-	expression tag
Tyr 465	-	expression tag
Phe 466	-	expression tag
Gln 467	-	expression tag
Gly 468	-	expression tag
His 469	-	expression tag
His 470	-	expression tag
His 471	-	expression tag
His 472	-	expression tag
His 473	-	expression tag
His 474	-	expression tag
His 475	-	expression tag
His 476	-	expression tag
His 477	-	expression tag
His 478	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	184215.1
Non-Polymers*	Number of molecules	3
	Total formula weight	196.2
All*	Total formula weight	184411.3

*Water molecules are not included.