8PSZ

Tilapia Lake Virus polymerase in vRNA elongation state with additional mode B promoter (transcriptase conformation)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Polymerase acidic protein (PA-like)	polymer	419	47780.5	1	UniProt (A0A142I7Z3)	Tilapia lake virus
2	B (B)	Putative PB1	polymer	519	57179.4	1	UniProt (A0A1Y9SHW4)	Tilapia lake virus
3	C (C)	RNA-dependent RNA polymerase	polymer	478	53782.1	1	UniProt (A0A7G3S745)	Tilapia lake virus
4	D, E, G (V, S, D)	5' vRNA end - vRNA loop (40-mer)	polymer	40	12736.5	3		Tilapia lake virus
5	F (P)	Transcription-like product	polymer	21	6675.0	1		Tilapia lake virus
6	H, L, M (A, C)	ZINC ION	non-polymer		65.4	3	Chemie (ZN)
7	I (B)	[(2~{R},3~{S},4~{R},5~{R})-5-(4-azanyl-2-oxidanylidene-pyrimidin-1-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-~{N}-[oxidanyl(phosphonooxy)phosphoryl]phosphonamidic acid	non-polymer		482.2	1	Chemie (A0I)
8	J, K (B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
9	N, O, P, Q, R... (A, B, C, V, S...)	water	water		18.0	109	Chemie (HOH)

Sequence modifications

C: 1 - 457 (UniProt: A0A7G3S745)

PDB	External Database	Details
Lys 391	Arg 391	conflict
Gly 458	-	expression tag
Ser 459	-	expression tag
Gly 460	-	expression tag
Ser 461	-	expression tag
Glu 462	-	expression tag
Asn 463	-	expression tag
Leu 464	-	expression tag
Tyr 465	-	expression tag
Phe 466	-	expression tag
Gln 467	-	expression tag
Gly 468	-	expression tag
His 469	-	expression tag
His 470	-	expression tag
His 471	-	expression tag
His 472	-	expression tag
His 473	-	expression tag
His 474	-	expression tag
His 475	-	expression tag
His 476	-	expression tag
His 477	-	expression tag
His 478	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	203626.6
Non-Polymers*	Number of molecules	6
	Total formula weight	727.0
All*	Total formula weight	204353.6

*Water molecules are not included.